Senthamarai Kannan Monika, Hari Haran Ponlakshmi S, Sundar Krishnan, Kunjiappan Selvaraj, Balakrishnan Vanavil
Department of Biotechnology, Kalasalingam Academy of Research and Education, Krishnankoil, Tamilnadu, 626126, India.
Prog Biomater. 2022 Jun;11(2):229-241. doi: 10.1007/s40204-022-00190-x. Epub 2022 May 27.
Recently the use of plant-derived extracts for the green synthesis of nanoparticles has drawn considerable attention. In the present study silver and copper nanoparticles were synthesized using extracts of Andrographis paniculata which is found to possess various pharmacological properties. The synthesized nanoparticles were characterized using UV spectroscopy, SEM with EDS, XRD, TEM and DLS. Furthermore, an attempt is made to impregnate these nanoparticles onto cotton bandages. The structure and morphology of silver nanoparticles impregnated onto the cotton bandages were confirmed by SEM. The anti-bacterial activity of cotton bandages loaded with silver and copper nanoparticles was tested against Escherichia coli, Bacillus cereus, and Staphylococcus aureus using a modified disc diffusion assay. The results indicate that the cotton bandages biofabricated with nanoparticles exhibited anti-bacterial activity in terms of zone of inhibition of growth of tested bacteria suggesting their usage as medical textiles in various biomedical applications for the prevention of infections. Hence, the nanoparticles impregnated cotton fibers can be applied for the development of masks, aprons, etc. to protect against bacterial penetration and as well to counteract the present situation of the world.
最近，利用植物提取物进行纳米粒子的绿色合成受到了广泛关注。在本研究中，使用具有多种药理特性的穿心莲提取物合成了银和铜纳米粒子。采用紫外光谱、扫描电子显微镜（SEM）结合能谱仪（EDS）、X射线衍射（XRD）、透射电子显微镜（TEM）和动态光散射（DLS）对合成的纳米粒子进行了表征。此外，还尝试将这些纳米粒子浸渍到棉绷带上。通过扫描电子显微镜确认了浸渍在棉绷带上的银纳米粒子的结构和形态。使用改良的纸片扩散法测试了负载银和铜纳米粒子的棉绷带对大肠杆菌、蜡样芽孢杆菌和金黄色葡萄球菌的抗菌活性。结果表明，用纳米粒子生物制造的棉绷带在测试细菌生长抑制区方面表现出抗菌活性，这表明它们可作为医用纺织品用于各种生物医学应用中以预防感染。因此，浸渍有纳米粒子的棉纤维可用于开发口罩、围裙等，以防止细菌渗透，并应对当前的全球形势。
