Karzi Vasiliki, Tzatzarakis Manolis N, Alegakis Athanasios, Vakonaki Elena, Fragkiadoulaki Irene, Kaloudis Konstantinos, Chalkiadaki Christina, Apalaki Paraskevi, Panagiotopoulou Maria, Kalliantasi Aikaterini, Kouretas Demetrios, Docea Anca Oana, Calina Daniela, Tsatsakis Aristidis
Center of Toxicology, Medicine School, University of Crete, 70013 Heraklion, Greece.
Department of Biochemistry and Biotechnology, University of Thessaly, Viopolis, Mezourlo, 41500 Larissa, Greece.
Toxics. 2022 May 12;10(5):246. doi: 10.3390/toxics10050246.
Recently, an increasing number of chemical compounds are being characterized as endocrine disruptors since they have been proven to interact with the endocrine system, which plays a crucial role in the maintenance of homeostasis. Glyphosate is the active substance of the herbicide Roundup, bisphenol A (BPA) and di (2-ethylhexyl) phthalate (DEHP) are used as plasticizers, while triclosan (TCS), methyl (MePB), propyl (PrPB), and butyl (BuPB) parabens are used as antimicrobial agents and preservatives mainly in personal care products. Studies indicate that exposure to these substances can affect humans causing developmental problems and problems in the endocrine, reproductive, nervous, immune, and respiratory systems. Although there are copious studies related to these substances, there are few in vivo studies related to combined exposure to these endocrine disruptors. The aim of the present pilot study is the investigation and assessment of the above substances' toxicity in rabbits after twelve months of exposure to glyphosate (both pure and commercial form) and to a mixture of all the above substances at subtoxic levels. The lack of data from the literature concerning rabbits' exposure to these substances and the restrictions of the 3Rs Principle will result in a limited number of animals available for use (four animals per group, twenty animals in total).
最近，越来越多的化合物被认定为内分泌干扰物，因为它们已被证明会与内分泌系统相互作用，而内分泌系统在维持体内平衡方面起着至关重要的作用。草甘膦是除草剂农达的活性成分，双酚A（BPA）和邻苯二甲酸二（2-乙基己基）酯（DEHP）用作增塑剂，而三氯生（TCS）、对羟基苯甲酸甲酯（MePB）、对羟基苯甲酸丙酯（PrPB）和对羟基苯甲酸丁酯（BuPB）主要用作个人护理产品中的抗菌剂和防腐剂。研究表明，接触这些物质会对人类产生影响，导致发育问题以及内分泌、生殖、神经、免疫和呼吸系统问题。尽管有大量关于这些物质的研究，但关于这些内分泌干扰物联合暴露的体内研究却很少。本初步研究的目的是在兔子接触草甘膦（纯品和商业制剂）以及上述所有物质的亚毒性水平混合物十二个月后，调查和评估这些物质的毒性。由于缺乏关于兔子接触这些物质的文献数据以及3R原则的限制，可供使用的动物数量有限（每组四只动物，总共二十只动物）。
