School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310018, PR China.
College of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou 310014, PR China.
Food Chem. 2022 Oct 15;391:133288. doi: 10.1016/j.foodchem.2022.133288. Epub 2022 May 21.
Natamycin was encapsulated in gliadin-casein nanoparticles (G-C NPs) to control black rot in cherry tomato against Alternaria alternata. The G-C NPs with a mean particle diameter of 211 ± 4 nm were prepared using an anti-solvent method. The composite NPs showed better stability against neutral pH, ion, and storage than gliadin NPs. The quenching of gliadin by natamycin was static with a constant of 5.99 × 10 M∙S, and was spontaneous with a free energy of -23.5 kJ∙M at 298 K. Both hydrophobic stacking and hydrogen bonds between natamycin and gliadin were found as the major driven force in the formation of the complex. The NPs kept the antifungal activity of natamycin with improved photostability. The NPs coatings exhibited better results than natamycin in controlling black rot on cherry tomato. This study shows the potential of the G-C NPs as all-natural delivery systems for natamycin in post-harvest treatments.
纳他霉素被包封在醇溶蛋白-酪蛋白纳米粒（G-C NPs）中，以控制樱桃番茄的炭疽病，防治链格孢菌。采用反溶剂法制备平均粒径为 211±4nm 的 G-C NPs。复合 NPs 比醇溶蛋白 NPs 具有更好的中性 pH 值、离子和储存稳定性。纳他霉素对醇溶蛋白的猝灭是静态的，在 298 K 时，常数为 5.99×10 M∙S，自由能为-23.5 kJ∙M。纳他霉素和醇溶蛋白之间的疏水堆积和氢键被发现是形成复合物的主要驱动力。纳米粒保持了纳他霉素的抗真菌活性，同时提高了光稳定性。纳米粒涂层在控制樱桃番茄炭疽病方面的效果优于纳他霉素。本研究表明，G-C NPs 作为一种天然的纳他霉素递送系统，具有在采后处理中应用的潜力。
