Fang Meihan, Wang Jialu, Fang Sheng, Zuo Xiaobo
School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310018, China.
School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310018, China.
Int J Biol Macromol. 2023 Aug 15;246:125685. doi: 10.1016/j.ijbiomac.2023.125685. Epub 2023 Jul 4.
In this study, gliadin-carboxymethyl chitosan composite nanoparticles (GC NPs) co-encapsulated natamycin (Nata) and theaflavins (TFs) were constructed and added as an antioxidant, antifungal, and structural enhancer to carboxymethyl chitosan (CMCS) films. The stabilized GC NPs with a particle size of 160.7 ± 2.8 nm, a zeta potential of -29.0 ± 0.9 mV, and a protein content in the supernatant of 96 ± 1 % could be fabricated. Tests of pH and salt ions showed that the stability of NPs dispersion was based on electrostatic repulsion. Co-encapsulation of TFs enhanced the photostability of Nata and the antioxidant activity of the NPs dispersion. The interactions between gliadin with Nata and TFs were studied by molecular simulations. As a functional additive, the addition of Nata/TFs-GC NPs could improve the optical properties, mechanical properties, water-blocking capability, and antifungal and antioxidant activities of the CMCS films. The in-vivo test showed that the functional film could be used to inhibit the growth of Aspergillus niger on cheese.
在本研究中，构建了共包封纳他霉素（Nata）和茶黄素（TFs）的麦醇溶蛋白-羧甲基壳聚糖复合纳米颗粒（GC NPs），并将其作为抗氧化剂、抗真菌剂和结构增强剂添加到羧甲基壳聚糖（CMCS）薄膜中。可以制备出粒径为160.7±2.8nm、ζ电位为-29.0±0.9mV且上清液中蛋白质含量为96±1%的稳定GC NPs。pH和盐离子测试表明，NPs分散体的稳定性基于静电排斥作用。TFs的共包封增强了Nata的光稳定性和NPs分散体的抗氧化活性。通过分子模拟研究了麦醇溶蛋白与Nata和TFs之间的相互作用。作为一种功能性添加剂，添加Nata/TFs-GC NPs可以改善CMCS薄膜的光学性能、机械性能、阻水能力以及抗真菌和抗氧化活性。体内试验表明，该功能薄膜可用于抑制黑曲霉在奶酪上的生长。
