Chen Chang, Ma Quantao, Jiang Jinzhu, Wang Tieshan, Qiu Linghui, Liu An
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China.
Brain Sci. 2022 Apr 28;12(5):572. doi: 10.3390/brainsci12050572.
Middle cerebral artery occlusion (MCAO), with the characteristics of high morbidity, high recurrence rate, high mortality, and disability rate, is a typical manifestation of ischemic stroke and has become a hot research topic in the clinical field. The protective effects of nuciferine on brain injury MCAO rats were investigated and its mechanisms of actions were revealed. The MCAO rats were established by the suture method. The pathological staining of the rat brain was processed and observed, the pharmacodynamics assay of nuciferine were studied, and the gene expression regulation by nuciferine was detected by transcriptome technology. The results showed that nuciferine significantly alleviated brain damage in MCAO rats, and the transcriptomic results suggested that nuciferine could exert therapeutic effects through the regulation of lipid metabolism, including arachidonic acid metabolism, sphingolipid metabolism, the PPAR signaling pathway and other related pathways. This finding provided new perspectives on the treatment of MCAO with nuciferine and facilitates the development of novel drugs for this disease.
大脑中动脉闭塞（MCAO）具有高发病率、高复发率、高死亡率和致残率的特点，是缺血性中风的典型表现，已成为临床领域的研究热点。本研究探讨了荷叶碱对MCAO大鼠脑损伤的保护作用，并揭示其作用机制。采用线栓法制备MCAO大鼠模型。对大鼠脑组织进行病理染色并观察，研究荷叶碱的药效学，通过转录组技术检测荷叶碱对基因表达的调控。结果表明，荷叶碱能显著减轻MCAO大鼠的脑损伤，转录组学结果提示荷叶碱可通过调节脂质代谢发挥治疗作用，包括花生四烯酸代谢、鞘脂代谢、PPAR信号通路等相关途径。这一发现为荷叶碱治疗MCAO提供了新的视角，有助于开发针对该疾病的新型药物。
