Quinteros María Fernanda, Martínez Jenny, Barrionuevo Alejandra, Rojas Marcelo, Carrillo Wilman
Posgrados, Universidad Técnica de Cotopaxi, Latacunga 050150, Ecuador.
Departamento de Investigación, Universidad Estatal de Bolívar, Guaranda 020102, Ecuador.
Biology (Basel). 2022 May 20;11(5):776. doi: 10.3390/biology11050776.
Edible insects can represent an alternative to obtain high-quality proteins with positive biological properties for human consumption. Cricket flour () was used to obtain cricket protein concentrate (CPC) using pHs (10.0 and 12.0) of extraction and pHs (3.0, 4.0, 5.0, and 6.0) of isoelectric precipitation (pI). Protein content, water and oil absorption capacity, protein solubility, antioxidant, and anti-inflammatory activities were determined. In addition, the protein profile was characterized by electrophoresis and the in vitro CPC digestibility was evaluated. Cricket flour presented 45.75% of protein content and CPC 12-5.0 presented a value of 71.16% protein content using the Dumas method. All samples were more soluble at pH 9.0 and 12.0. CPC 12-3.0 presented a percentage of water-binding capacity (WBC) of 41.25%. CPC 12-6.0 presented a percentage of oil-binding capacity (OBC) of 72.93%. All samples presented a high antioxidant and anti-inflammatory activity. CPC 12-4.0 presented a value FRAP of 70,034 umol trolox equivalents (TE)/g CPC, CPC 12-6.0 presented a value ABTS of 124,300 umol TE/g CPC and CPC 10-3.0 presented a DPPH value of 68,009 umol TE/g CPC. CPC 10-6.0 and CPC 12-6.0 presented high anti-inflammatory activity, with values of 93.55% and 93.15% of protection, respectively. CPCs can be used as functional ingredients in the food industry for their excellent functional and biological properties.
可食用昆虫可以作为一种替代物，用于获取对人类食用具有积极生物学特性的优质蛋白质。使用提取pH值（10.0和12.0）和等电沉淀pH值（3.0、4.0、5.0和6.0）从蟋蟀粉中获得蟋蟀浓缩蛋白（CPC）。测定了蛋白质含量、水和油吸收能力、蛋白质溶解度、抗氧化和抗炎活性。此外，通过电泳对蛋白质谱进行了表征，并评估了体外CPC的消化率。采用杜马斯法，蟋蟀粉的蛋白质含量为45.75%，CPC 12 - 5.0的蛋白质含量为71.16%。所有样品在pH 9.0和12.0时更易溶解。CPC 12 - 3.0的水结合能力（WBC）百分比为41.25%。CPC 12 - 6.0的油结合能力（OBC）百分比为72.93%。所有样品均具有较高的抗氧化和抗炎活性。CPC 12 - 4.0的铁还原抗氧化能力（FRAP）值为70,034 μmol Trolox当量（TE）/g CPC，CPC 12 - 6.0的2,2'-联氮-双-3-乙基苯并噻唑啉-6-磺酸（ABTS）值为124,300 μmol TE/g CPC，CPC 10 - 3.0的二苯基苦味酰基自由基（DPPH）值为68,009 μmol TE/g CPC。CPC 10 - 6.0和CPC 12 - 6.0具有较高的抗炎活性，保护值分别为93.55%和93.15%。由于其优异的功能和生物学特性，CPC可作为食品工业中的功能成分。
