Callesen Katrine T, Mogren Sofia, Berlin Frida, Andersson Cecilia, Schmidt Susanne, Klitfod Lotte, Esteban Vanesa, Poulsen Lars K, Jensen Bettina M
Laboratory of Medical Allergology, Copenhagen University Hospital, Gentofte, 2900 Hellerup, Denmark.
Respiratory Cell Biology, Department of Experimental Medical Science, Lund University, 22184 Lund, Sweden.
Biomedicines. 2022 May 3;10(5):1062. doi: 10.3390/biomedicines10051062.
Mast cells (MCs) are distributed in tissues throughout the body and are highly involved in many physiological and pathophysiological processes. The potential and involvement of different MC phenotypes are still not well understood. MCs are present in blood vessel walls, but their specific phenotypic features are unknown. We aimed at characterizing MCs from human saphenous veins for localization, mediator content, and receptor expression. This was done in MCs from both healthy and varicose human saphenous veins (hSV and vSV, respectively). For both vSV and hSV, we found that vein MCs are mainly present in the tunica adventitia (99% MCs in adventitia) and that the population consists of both MC and MC phenotypes (vSV: 55% MC, hSV: 64% MC). The vein MCs contained high levels of histamine (vSV: 27 pg/MC, hSV: 55 pg/MC) and tryptase (vSV: 98 pg/MC, hSV: 111 pg/MC), indicating a strong potential for regulatory effects on blood vessels. The receptor expression of FcεRI, MRGPRX2, PTAFR, C3aR, and C5aR was found, even though the percentage of positive cells differed between vSV and hSV MCs. We conclude that vein MCs from the blood vessel wall have a high potential to affect the tissue around them.
肥大细胞（MCs）分布于全身组织，高度参与许多生理和病理生理过程。不同MC表型的潜能和参与情况仍未得到充分了解。MCs存在于血管壁中，但其具体表型特征尚不清楚。我们旨在对来自人隐静脉的MCs进行定位、介质含量和受体表达方面的特征描述。这一研究在来自健康和曲张人隐静脉（分别为hSV和vSV）的MCs中进行。对于vSV和hSV，我们发现静脉MCs主要存在于外膜（外膜中99%为MCs），且该群体由MC和MC表型组成（vSV：55%为MC，hSV：64%为MC）。静脉MCs含有高水平的组胺（vSV：27 pg/MC，hSV：55 pg/MC）和类胰蛋白酶（vSV：98 pg/MC，hSV：111 pg/MC），表明其对血管具有强大的调节作用潜能。尽管vSV和hSV MCs中阳性细胞的百分比有所不同，但仍发现了FcεRI、MRGPRX2、PTAFR、C3aR和C5aR的受体表达。我们得出结论，血管壁中的静脉MCs具有影响其周围组织的高潜能。
