Karas Adam, Holmannova Drahomira, Borsky Pavel, Fiala Zdenek, Andrys Ctirad, Hamakova Kvetoslava, Svadlakova Tereza, Palicka Vladimir, Krejsek Jan, Rehacek Vit, Esterkova Monika, Kovarikova Helena, Borska Lenka
Institute of Preventive Medicine, Faculty of Medicine in Hradec Kralove, Charles University, 500 03 Hradec Kralove, Czech Republic.
Institute of Clinical Immunology and Allergology, University Hospital, Faculty of Medicine in Hradec Kralove, Charles University, 500 03 Hradec Kralove, Czech Republic.
Biomedicines. 2022 May 13;10(5):1133. doi: 10.3390/biomedicines10051133.
This study aims to investigate potential markers of psoriasis and aging, and to elucidate possible connections between these two processes.
The serum samples of 60 psoriatic patients and 100 controls were analysed, and the levels of four selected parameters (AGEs, RAGE, NAD, and elastin) were determined using commercial ELISA kits. Serum C-reactive protein was assayed using an immune-nephelometry method.
Among the patients, the levels of CRP, AGEs, and RAGE were all increased, while the levels of NAD were reduced when compared to the control group. A negative correlation between the levels of AGEs and NAD was found. A negative correlation between age and the NAD levels among the control group was observed, however among the patients the relationship was diminished. While there was no difference in the levels of native elastin between the patients and the controls, a positive correlation between the levels of native elastin and age and a negative correlation between the levels of native elastin and the severity of psoriasis were found.
The results of our study support the notion of psoriasis and possibly other immune-mediated diseases accelerating the aging process through sustained systemic damage. The serum levels of CRP, NAD, AGEs, and RAGE appear to be promising potential biomarkers of psoriasis. The decrease in the serum levels of NAD is associated with (pro)inflammatory states. Our analysis indicates that the levels of native elastin might strongly reflect both the severity of psoriasis and the aging process.
本研究旨在探究银屑病和衰老的潜在标志物，并阐明这两个过程之间可能存在的联系。
分析了60例银屑病患者和100例对照者的血清样本，使用商用酶联免疫吸附测定试剂盒测定了四个选定参数（晚期糖基化终末产物、晚期糖基化终末产物受体、烟酰胺腺嘌呤二核苷酸和弹性蛋白）的水平。采用免疫比浊法测定血清C反应蛋白。
与对照组相比，患者组中C反应蛋白、晚期糖基化终末产物和晚期糖基化终末产物受体的水平均升高，而烟酰胺腺嘌呤二核苷酸水平降低。发现晚期糖基化终末产物水平与烟酰胺腺嘌呤二核苷酸水平呈负相关。在对照组中观察到年龄与烟酰胺腺嘌呤二核苷酸水平呈负相关，但在患者组中这种关系减弱。虽然患者和对照者之间天然弹性蛋白水平没有差异，但发现天然弹性蛋白水平与年龄呈正相关，与银屑病严重程度呈负相关。
我们的研究结果支持银屑病以及可能其他免疫介导疾病通过持续的全身损伤加速衰老过程这一观点。血清C反应蛋白、烟酰胺腺嘌呤二核苷酸、晚期糖基化终末产物和晚期糖基化终末产物受体水平似乎是银屑病有前景的潜在生物标志物。烟酰胺腺嘌呤二核苷酸血清水平的降低与（促）炎状态相关。我们的分析表明，天然弹性蛋白水平可能强烈反映银屑病的严重程度和衰老过程。
