Zhang Yigong, Zhou Jiyang, Zhang Yi, Zhang Daoyuan
Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830017, China.
Xinjiang Key Laboratory of Conservation and Utilization of Plant Gene Resources, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, China.
Genes (Basel). 2022 Apr 20;13(5):718. doi: 10.3390/genes13050718.
The ubiquitination pathway has been found to regulate plant responses to environmental stress. However, the role of E3 ubiquitin ligase in desiccation tolerant moss has not yet been elucidated. Previous research has shown that the abscisic acid (ABA) signaling factor ScABI3 can significantly increase desiccation tolerance and reduce ABA sensitivity in the desert moss . In this study, we identified a RING-type E3 ubiquitin ligase, ScPRT1, and showed that ScABI3 can directly interact with ScPRT1 in vitro and in vivo. Furthermore, we found that the high expression of can interfere with the transcription of under ABA treatment. Therefore, we speculate that ScPRT1 may degrade ScABI3 through the ubiquitin-26S proteasome system and participate in ABA-dependent signaling in response to ABA-insensitivity or desiccation tolerance in . The findings from our study may enrich our knowledge of the role of E3 ubiquitin ligase in desiccation tolerance and lay a theoretical foundation for an in-depth study of the relationship between ubiquitination modification and ABA signal transduction under environmental stress.
泛素化途径已被发现可调节植物对环境胁迫的反应。然而，E3泛素连接酶在耐旱苔藓中的作用尚未阐明。先前的研究表明，脱落酸（ABA）信号因子ScABI3可显著提高沙漠苔藓的耐旱性并降低其对ABA的敏感性。在本研究中，我们鉴定出一种RING型E3泛素连接酶ScPRT1，并表明ScABI3在体外和体内均可直接与ScPRT1相互作用。此外，我们发现ScPRT1的高表达可在ABA处理下干扰ScABI3的转录。因此，我们推测ScPRT1可能通过泛素-26S蛋白酶体系统降解ScABI3，并参与响应沙漠苔藓中ABA不敏感或耐旱性的ABA依赖性信号传导。我们的研究结果可能会丰富我们对E3泛素连接酶在耐旱性中作用的认识，并为深入研究环境胁迫下泛素化修饰与ABA信号转导之间的关系奠定理论基础。
