Department of Spine Disorders and Pediatric Orthopedics, Faculty of Medicine, Poznan University of Medical Sciences, 28 Czerwca 1956 r. Street 135/147, 61-545 Poznan, Poland.
Chair and Department of Cell Biology, Faculty of Health Sciences, Poznan University of Medical Sciences, Rokietnicka 5D, 60-806 Poznan, Poland.
Genes (Basel). 2022 Apr 22;13(5):739. doi: 10.3390/genes13050739.
The study aimed to detect the presence and assess the expression levels of the estrogen receptors type 1 (ESR1) and type 2 (ESR2) within paravertebral skeletal muscles of female patients with idiopathic scoliosis (IS) in relation to phenotype parameters. Intraoperatively, the muscle samples were obtained from 35 adolescent females. The RT-qPCR, western blot and immunohistochemistry techniques were applied. The ESR1 and ESR2 were detected within paravertebral skeletal muscle cells, either the superficial or the deep ones. The expression level was significantly higher in the deep muscles compared to the superficial ones. A left-right asymmetry of the and expression level was demonstrated in the deep muscles. There was a significant relationship between the expression asymmetry and either the Cobb angle or the progression risk factor: both parameters decreased to the smallest values in the case of symmetric or expression, while they increased with increasing expression asymmetry. In conclusion, the ESR1 and ESR2 presence was confirmed in skeletal paravertebral muscles of patients with idiopathic scoliosis. The increased expression level and asymmetry of estrogen receptors in deep skeletal muscles was related to increasing scoliotic deformity magnitude or increasing risk of deformity deterioration. These findings may highlight the etiopathogenesis of IS in children.
本研究旨在检测特发性脊柱侧凸（IS）女性患者椎旁骨骼肌中雌激素受体 1（ESR1）和雌激素受体 2（ESR2）的存在，并评估其与表型参数的关系。术中从 35 名青春期女性患者中获取肌肉样本。应用 RT-qPCR、western blot 和免疫组织化学技术进行检测。ESR1 和 ESR2 存在于椎旁骨骼肌细胞中，无论是浅层还是深层。与浅层相比，深层肌肉中 ESR1 和 ESR2 的表达水平显著更高。在深层肌肉中观察到 和 表达水平的左右不对称。 表达的不对称性与 Cobb 角或进展风险因素之间存在显著关系：在 或 表达对称的情况下，这两个参数降低到最小，而随着 表达不对称性的增加而增加。总之，在特发性脊柱侧凸患者的椎旁骨骼肌中证实了 ESR1 和 ESR2 的存在。在深层骨骼肌中，雌激素受体的表达水平增加和不对称性与脊柱侧凸畸形程度的增加或畸形恶化风险的增加有关。这些发现可能突出了儿童特发性脊柱侧凸的病因发病机制。
