Skibinska Izabela, Tomaszewski Marek, Andrusiewicz Miroslaw, Urbaniak Paulina, Czarnecka-Klos Roza, Shadi Milud, Kotwicki Tomasz, Kotwicka Malgorzata
Department of Cell Biology, Health Sciences Faculty, University of Medical Sciences, Poznan, Poland.
Spine Disorders and Pediatric Orthopedics Department, Faculty of Medicine I, University of Medical Sciences, Poznan, Poland.
PLoS One. 2016 Apr 5;11(4):e0152286. doi: 10.1371/journal.pone.0152286. eCollection 2016.
The aim of this study was to detect and assess the estrogen receptor (ESR) coactivator PELP1 expression within human paraspinal skeletal muscles in patients suffering from idiopathic scoliosis.
During surgical correction of scoliosis the muscle biopsies harvested in 29 females. Presence of PELP1, ESR1 and ESR2 genes transcripts was studied using RT-qPCR technique while immunohistochemistry and western blot methods were used to detect the PEPL1 protein presence.
PELP1 expression in deep paraspinal muscles revealed higher than in superficial back muscles (p = 0.005). Positive immunohistochemical staining for PELP1 was observed in the nuclei of the paraspinal muscle cells. Western blot revealed PELP1 protein in all samples. No significant difference in PELP1 expression between the convex and the concave scoliosis side (p>0.05) was found. In deep paraspinal back muscles, a significant correlation between the PELP1 expression level on the concave side and the Cobb angle (r = 0.4; p<0.05) was noted as well as between the PELP1 and ESR1 expression level (r = 0.7; p<0.05) while no correlation between PELP1 and ESR2 expression level was found.
To our knowledge, three techniques for the first time demonstrated the presence of the PELP1 in paraspinal muscles of patients with idiopathic scoliosis. The PELP1 potential regulatory impact on back muscle function is to be further investigated.
本研究旨在检测和评估特发性脊柱侧凸患者椎旁骨骼肌中雌激素受体（ESR）共激活因子PELP1的表达情况。
在脊柱侧凸手术矫正过程中，采集了29名女性的肌肉活检样本。采用RT-qPCR技术研究PELP1、ESR1和ESR2基因转录本的存在情况，同时采用免疫组织化学和蛋白质印迹法检测PEPL1蛋白的存在情况。
椎旁深层肌肉中PELP1的表达高于背部浅层肌肉（p = 0.005）。在椎旁肌细胞核中观察到PELP1的免疫组织化学阳性染色。蛋白质印迹法显示所有样本中均有PELP1蛋白。未发现脊柱侧凸凸侧和凹侧之间PELP1表达有显著差异（p>0.05）。在椎旁深层背部肌肉中，凹侧PELP1表达水平与Cobb角之间存在显著相关性（r = 0.4；p<0.05），PELP1与ESR1表达水平之间也存在显著相关性（r = 0.7；p<0.05），而未发现PELP1与ESR2表达水平之间存在相关性。
据我们所知，三种技术首次证明了特发性脊柱侧凸患者椎旁肌肉中存在PELP1。PELP1对背部肌肉功能的潜在调节作用有待进一步研究。
