College of Horticulture, Shenyang Agricultural University, Shenyang 110866, China.
Center for Plant Science Innovation and Department of Biochemistry, University of Nebraska-Lincoln, Lincoln, NE 68588, USA.
Int J Mol Sci. 2022 May 11;23(10):5369. doi: 10.3390/ijms23105369.
is a soil-borne pathogen that attacks the roots of cruciferous plants and causes clubroot disease. CircRNAs are noncoding RNAs, widely existing in plant and animal species. Although knowledge of circRNAs has been updated continuously and rapidly, information about circRNAs in the regulation of clubroot disease resistance is extremely limited in . Here, Chinese cabbage (BJN 222) containing clubroot resistance genes () against . Pb4 was susceptible to PbE. To investigate the mechanism of cicRNAs responsible for clubroot disease resistance in . , circRNA-seq was performed with roots of 'BJN 222' at 0, 8, and 23 days post-inoculated (dpi) with Pb4 and PbE. A total of 231 differentially expressed circRNAs were identified between the groups. Based on the differentially expressed circRNAs, the circRNA-miRNA-mRNA network was constructed using the target genes directly or indirectly related to plant resistance. Upregulated suppressed the expression of , leading to the upregulation of , which might cause plant resistance. Our results provide new insights into clubroot resistance mechanisms and lay a foundation for further studies exploring complex gene regulation networks in . .
是一种土传病原体，攻击十字花科植物的根部，导致根肿病。CircRNAs 是非编码 RNA，广泛存在于动植物物种中。尽管对 circRNAs 的了解不断更新且迅速，但在 中，关于 circRNAs 在调节根肿病抗性方面的信息极为有限。在这里，含有根肿病抗性基因（）的白菜（BJN 222）对. Pb4 具有抗性，而对 PbE 则易感。为了研究负责. 的 cicRNAs 抵抗根肿病的机制，我们对用 Pb4 和 PbE 接种 0、8 和 23 天的 'BJN 222' 的根进行了 circRNA-seq。在两组之间共鉴定出 231 个差异表达的 circRNAs。基于差异表达的 circRNAs，使用与植物抗性直接或间接相关的靶基因构建了 circRNA-miRNA-mRNA 网络。上调的 抑制了 的表达，导致 的上调，这可能导致植物抗性。我们的结果为根肿病抗性机制提供了新的见解，并为进一步研究 中的复杂基因调控网络奠定了基础。
