Department of Agricultural, Food and Nutritional Science, University of Alberta, 4-10 Agriculture/Forestry Centre, Edmonton, AB, T6G 2P5, Canada.
Mol Plant Microbe Interact. 2019 Oct;32(10):1360-1377. doi: 10.1094/MPMI-12-18-0341-R. Epub 2019 Aug 15.
Clubroot disease, caused by Woronin, is a major threat to the production of crops. Resistance to different pathotypes has been reported in the A genome, chromosome A08; however, the molecular mechanism of this resistance, especially the involvement of long noncoding RNAs (lncRNAs), is not understood. We have used a strand-specific lncRNA-Seq approach to catalog lncRNAs from the roots of clubroot-susceptible and -resistant lines. In total, 530 differentially expressed (DE) lncRNAs were identified, including 88% of long intergenic RNAs and 11% natural antisense transcripts. Sixteen lncRNAs were identified as target mimics of the microRNAs (miRNAs) and eight were identified as the precursors of miRNAs. KEGG pathway analysis of the DE lncRNAs showed that the cis-regulated target genes mostly belong to the phenylpropanoid biosynthetic pathway (15%) and plant-pathogen interactions (15%) while the transregulated target genes mostly belong to carbon (18%) and amino acid biosynthesis pathway (19%). In all, 24 DE lncRNAs were identified from chromosome A08, which is known to harbor a quantitative trait locus conferring resistance to different pathotypes; however, eight of these lncRNAs showed expression only in the resistant plants. These results could form the basis for future studies aimed at delineating the roles of lncRNAs in plant-microbe interactions.
根肿病，由 Woronin 引起，是作物生产的主要威胁。在 A 基因组、染色体 A08 中已报道对不同 菌系的抗性；然而，这种抗性的分子机制，特别是长非编码 RNA（lncRNA）的参与，尚不清楚。我们使用了一种链特异性 lncRNA-Seq 方法，从感病和抗病 系的根中对 lncRNA 进行编目。总共鉴定出 530 个差异表达（DE）lncRNA，包括 88%的长基因间 RNA 和 11%的天然反义转录本。鉴定出 16 个 lncRNA 作为 miRNA 的靶模拟物，8 个作为 miRNA 的前体。DE lncRNA 的 KEGG 通路分析表明，顺式调控靶基因主要属于苯丙烷生物合成途径（15%）和植物-病原体相互作用（15%），而反式调控靶基因主要属于碳（18%）和氨基酸生物合成途径（19%）。总共从已知含有赋予对不同 菌系抗性的数量性状位点的染色体 A08 中鉴定出 24 个 DE lncRNA；然而，其中 8 个 lncRNA 仅在抗病植物中表达。这些结果可能为未来研究植物-微生物相互作用中 lncRNA 的作用奠定基础。
