The National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI), Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Department of Biology, Southern University of Science and Technology, Shenzhen 518055, China.
Int J Mol Sci. 2022 May 12;23(10):5394. doi: 10.3390/ijms23105394.
CONSTITUTIVE PHOTOMORPHOGENIC 1 (COP1) is the key photomorphogenic inhibitor that has been extensively studied in higher plants. Nevertheless, its role has not been documented in the economically important soybean. Here we investigated the functions of two homologous genes, and , by analyzing and mutants with indels using CRISPR in soybean. We revealed that, although both genes are required for skotomorphogenesis in the dark, the gene seems to play a more prominent role than in promoting stem elongation under normal light conditions. Consistently, the bZIP transcriptional factors STF1/2, which repress stem elongation in soybean, accumulated to the highest level in the double mutant, followed by the and mutants. Furthermore, the mutants showed reduced shade response and enhanced performance under high-density conditions in field trials. Taken together, this study provides essential genetic resources for elucidating functional mechanisms of GmCOP1 and breeding of high yield soybean cultivars for future sustainable agriculture.
组成型光形态建成 1 型（COP1）是高等植物中广泛研究的关键光形态建成抑制剂。然而，其在经济上重要的大豆中的作用尚未被记录。在这里，我们通过在大豆中使用 CRISPR 分析具有插入/缺失的 和 突变体，研究了两个同源基因 和 的功能。我们揭示，尽管这两个基因在黑暗中都需要进行黄化苗形成，但 基因似乎比 基因在正常光照条件下更能促进茎伸长。一致地，bZIP 转录因子 STF1/2 在大豆中抑制茎伸长，在 双突变体中积累到最高水平，其次是 和 突变体。此外， 突变体在田间试验中表现出对遮荫的反应降低和在高密度条件下的表现增强。总之，这项研究为阐明 GmCOP1 的功能机制以及为未来可持续农业培育高产大豆品种提供了重要的遗传资源。
