Department of Molecular Biology, Dankook University, Cheonan 31116, Korea.
Department of Bioconvergence Engineering, Research Institute of Advanced Omics, Dankook University, Yongin 16890, Korea.
Int J Mol Sci. 2022 May 12;23(10):5399. doi: 10.3390/ijms23105399.
Viral infections can be fatal and consequently, they are a serious threat to human health. Therefore, the development of vaccines and appropriate antiviral therapeutic agents is essential. Depending on the virus, it can cause an acute or a chronic infection. The characteristics of viruses can act as inhibiting factors for the development of appropriate treatment methods. Genome editing technology, including the use of clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated (Cas) proteins, zinc-finger nucleases (ZFNs), and transcription activator-like effector nucleases (TALENs), is a technology that can directly target and modify genomic sequences in almost all eukaryotic cells. The development of this technology has greatly expanded its applicability in life science research and gene therapy development. Research on the use of this technology to develop therapeutics for viral diseases is being conducted for various purposes, such as eliminating latent infections or providing resistance to new infections. In this review, we will look at the current status of the development of viral therapeutic agents using genome editing technology and discuss how this technology can be used as a new treatment approach for viral diseases.
病毒感染可能是致命的，因此对人类健康构成严重威胁。因此，开发疫苗和适当的抗病毒治疗药物是必不可少的。根据病毒的不同，它可能导致急性或慢性感染。病毒的特性可能成为开发适当治疗方法的抑制因素。基因组编辑技术，包括使用簇状规律间隔短回文重复序列（CRISPR）-CRISPR 相关（Cas）蛋白、锌指核酸酶（ZFNs）和转录激活因子样效应核酸酶（TALENs），是一种可以直接靶向和修饰几乎所有真核细胞基因组序列的技术。这项技术的发展极大地扩展了其在生命科学研究和基因治疗开发中的适用性。为了各种目的，如消除潜伏感染或提供对新感染的抗性，正在研究使用这项技术开发病毒疾病治疗药物。在这篇综述中，我们将探讨使用基因组编辑技术开发病毒治疗药物的现状，并讨论这项技术如何用作病毒疾病的新治疗方法。
