College of Life Sciences, Northeast Agricultural University, Harbin 150030, China.
College of Marine Life Sciences, Ocean University of China, Qingdao 266100, China.
Int J Mol Sci. 2022 May 20;23(10):5708. doi: 10.3390/ijms23105708.
Fragrant woodfern () is a medicinal plant rich in terpenoids. Ultraviolet-B (UV-B) light could increase concentration of terpenoids. The aim of this study was to analyze how UV-B regulates the terpenoid synthesis of the molecular regulatory mechanism in fragrant woodfern. In this study, compared with the control group, the content of the terpenes was significantly higher in fragrant woodfern leaves under UV-B treatment for 4 days (d). In order to identify how UV-B regulates the terpenoid metabolic mechanism in fragrant woodfern, we examined the mRNAs and small RNAs in fragrant woodfern leaves under UV-B treatment. mRNA and miRNA-seq identified 4533 DEGs and 17 DEMs in the control group compared with fragrant woodfern leaves under UV-B treatment for 4 d. mRNA-miRNA analysis identified miRNA target gene pairs consisting of 8 DEMs and 115 miRNAs. The target genes were subjected to GO and KEGG analyses. The results showed that the target genes were mainly enriched in diterpene biosynthesis, terpenoid backbone biosynthesis, plant hormone signal transduction, MEP pathway and MVA pathway, in which miR156 and miR160 regulate these pathways by targeting and , respectively. The mRNA and miRNA datasets identified a subset of candidate genes. It provides the theoretical basis that UV-B regulates the terpenoid synthesis of the molecular regulatory mechanism in fragrant woodfern.
香羊齿（）是一种富含萜类化合物的药用植物。中波紫外线（UV-B）可增加萜类化合物的浓度。本研究旨在分析 UV-B 如何调节香羊齿萜类合成的分子调控机制。在这项研究中，与对照组相比，在 UV-B 处理 4 天（d）后，香羊齿叶片中的萜类化合物含量显著升高。为了确定 UV-B 如何调节香羊齿萜类代谢机制，我们检测了 UV-B 处理下香羊齿叶片中的 mRNAs 和小 RNA。mRNA 和 miRNA-seq 鉴定出对照组与 UV-B 处理 4d 后香羊齿叶片中 4533 个差异表达基因（DEGs）和 17 个差异表达 microRNA（DEMs）。mRNA-miRNA 分析鉴定出由 8 个 DEM 和 115 个 miRNA 组成的 miRNA 靶基因对。对靶基因进行 GO 和 KEGG 分析。结果表明，靶基因主要富集在二萜生物合成、萜类骨架生物合成、植物激素信号转导、MEP 途径和 MVA 途径，其中 miR156 和 miR160 通过靶向和分别调控这些途径。mRNA 和 miRNA 数据集确定了一组候选基因。它为 UV-B 调节香羊齿萜类合成的分子调控机制提供了理论依据。
