Institute of Functional and Clinical Anatomy, Friedrich-Alexander-Universität Erlangen- Nuremberg, Universitätsstr. 19, 91054 Erlangen, Germany.
J. Heyrovský Institute of Physical Chemistry, Czech Academy of Sciences, 182 00 Prague, Czech Republic.
Int J Mol Sci. 2022 May 21;23(10):5783. doi: 10.3390/ijms23105783.
The ocular surface is in constant interaction with the environment and with numerous pathogens. Therefore, complex mechanisms such as a stable tear film and local immune defense mechanisms are required to protect the eye. This study describes the detection, characterization, and putative role of surfactant protein G (SP-G/SFTA2) with respect to wound healing and surface activity. Bioinformatic, biochemical, and immunological methods were combined to elucidate the role of SP-G in tear film. The results show the presence of SP-G in ocular surface tissues and tear film (TF). Increased expression of SP-G was demonstrated in TF of patients with dry eye disease (DED). Addition of recombinant SP-G in combination with lipids led to an accelerated wound healing of human corneal cells as well as to a reduction of TF surface tension. Molecular modeling of TF suggest that SP-G may regulate tear film surface tension and improve its stability through specific interactions with lipids components of the tear film. In conclusion, SP-G is an ocular surface protein with putative wound healing properties that can also reduce the surface tension of the tear film.
眼表面与环境和多种病原体不断相互作用。因此，需要稳定的泪膜和局部免疫防御机制等复杂机制来保护眼睛。本研究描述了表面活性剂蛋白 G（SP-G/SFTA2）在伤口愈合和表面活性方面的检测、特征和可能的作用。本研究结合生物信息学、生化和免疫学方法来阐明 SP-G 在泪膜中的作用。结果表明 SP-G 存在于眼表面组织和泪膜（TF）中。干眼症（DED）患者的 TF 中显示出 SP-G 表达增加。添加重组 SP-G 与脂质结合可加速人角膜细胞的伤口愈合，并降低 TF 表面张力。TF 的分子建模表明 SP-G 可能通过与泪膜脂质成分的特定相互作用来调节泪膜表面张力并提高其稳定性。总之，SP-G 是一种具有潜在伤口愈合特性的眼表面蛋白，也可以降低泪膜的表面张力。
