Department of Functional and Clinical Anatomy, Friedrich-Alexander-University Erlangen-Nürnberg (FAU), Erlangen, Germany.
Department of Ophthalmology, Friedrich-Alexander-University Erlangen-Nürnberg (FAU), Erlangen, Germany.
Sci Rep. 2018 Jun 28;8(1):9791. doi: 10.1038/s41598-018-28005-9.
The study aimed to characterize the expression and function of SFTA3 at the ocular surface and in tears. Ocular tissues, conjunctival (HCjE) and human corneal (HCE) epithelial cell lines as well as tearfilm of patients suffering from different forms of dry eye disease (DED) were analyzed by means of RT-PCR, western blot, immunohistochemistry, and ELISA. A possible role of recombinant SFTA3 in corneal wound healing was investigated performing in vitro scratch assays. Tear film regulatory properties were analyzed with the spinning drop method and the regulation of SFTA3 transcripts was studied in HCE and HCjE after incubation with proinflammatory cytokines as well as typical ocular pathogens by real-time RT-PCR and ELISA. The results reveal that human ocular tissue as well as tears of healthy volunteers express SFTA3 whereas tears from patients with DED showed significantly increased SFTA3 levels. In vitro wounding of HCE cell cultures that had been treated with recombinant SFTA3 demonstrated a significantly increased wound closure rate and rSFTA3 reduced the surface tension of tear fluid. The results indicate that SFTA3 at the ocular surface seemed to be involved in wound healing and the reduction of surface tension.
本研究旨在描述 SFTA3 在眼表和泪液中的表达和功能。通过 RT-PCR、Western blot、免疫组织化学和 ELISA 分析了眼部组织、结膜（HCjE）和人角膜（HCE）上皮细胞系以及患有不同类型干眼症（DED）患者的泪膜。通过体外划痕实验研究了重组 SFTA3 在角膜伤口愈合中的可能作用。通过旋转液滴法分析了泪膜调节特性，并通过实时 RT-PCR 和 ELISA 研究了 HCE 和 HCjE 在孵育促炎细胞因子和典型眼部病原体后 SFTA3 转录物的调节。结果表明，人眼组织以及健康志愿者的泪液中均表达 SFTA3，而 DED 患者的泪液中 SFTA3 水平显著升高。用重组 SFTA3 处理的 HCE 细胞培养物的体外划痕实验表明，伤口闭合率显著增加，rSFTA3 降低了泪液的表面张力。结果表明，眼表的 SFTA3 似乎参与了伤口愈合和表面张力的降低。
