Bandyopadhyay Prasun, Yadav Bal Govind, Kumar Srinivasan Ganesh, Kumar Rahul, Kogel Karl-Heinz, Kumar Shashi
International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi 110067, India.
Institute for Phytopathology, Justus Liebig University, Heinrich-Buff-Ring 26, D-35392 Giessen, Germany.
J Fungi (Basel). 2022 Apr 27;8(5):453. doi: 10.3390/jof8050453.
The soil microbiome contributes to nutrient acquisition and plant adaptation to numerous biotic and abiotic stresses. Numerous studies have been conducted over the past decade showing that plants take up nutrients better when associated with fungi and additional beneficial bacteria that promote plant growth, but the mechanisms by which the plant host benefits from this tripartite association are not yet fully understood. In this article, we report on a synergistic interaction between rice (), (an endophytic fungus colonizing the rice roots), and strain W5, a free-living nitrogen-fixing bacterium. On the basis of mRNA expression analysis and enzymatic activity, we found that co-inoculation of plant roots with the fungus and the rhizobacterium leads to enhanced plant growth and improved nutrient uptake compared to inoculation with either of the two microbes individually. Proteome analysis of further revealed that proteins involved in nitrogen and phosphorus metabolism are upregulated and improve nitrogen and phosphate uptake. Our results also show that supports colonization of rice roots by , and consequentially, the plants are more resistant to biotic stress upon co-colonization. Our research provides detailed insights into the mechanisms by which microbial partners synergistically promote each other in the interaction while being associated with the host plant.
土壤微生物群落有助于植物获取养分并适应多种生物和非生物胁迫。在过去十年中，已经进行了大量研究，结果表明，与促进植物生长的真菌和其他有益细菌共生时，植物能够更好地吸收养分，但植物宿主从这种三方共生关系中获益的机制尚未完全了解。在本文中，我们报道了水稻、（一种定殖于水稻根部的内生真菌）和自由生活的固氮细菌W5菌株之间的协同相互作用。基于mRNA表达分析和酶活性，我们发现，与单独接种这两种微生物之一相比，将真菌和根际细菌共同接种到植物根部可促进植物生长并改善养分吸收。对的蛋白质组分析进一步表明，参与氮和磷代谢的蛋白质上调，从而改善了氮和磷的吸收。我们的结果还表明，支持在水稻根部定殖，因此，共同定殖时植物对生物胁迫的抵抗力更强。我们的研究详细深入地揭示了微生物伙伴在与宿主植物共生时相互协同促进的机制。
