Arora Monika, Saxena Parul, Choudhary Devendra Kumar, Abdin Malik Zainul, Varma Ajit
Amity Institute of Microbial Technology (AIMT), Block 'E-3', 4th Floor, Amity University Campus, Sector-125, Gautam Buddha Nagar, Noida, UP, 201313, India.
Centre for Transgenic Plant Development, Faculty of Science, Hamdard University, New Delhi, India.
World J Microbiol Biotechnol. 2016 Feb;32(2):19. doi: 10.1007/s11274-015-1972-5. Epub 2016 Jan 8.
At present, Artemisia annua L. is the major source of artemisinin production. To control the outbreaks of malaria, artemisinin combination therapies (ACTs) are recommended, and hence an ample amount of artemisinin is required for ACTs manufacture to save millions of lives. The low yield of this antimalarial drug in A. annua L. plants (0.01-1.1%) ensues its short supply and high cost, thus making it a topic of scrutiny worldwide. In this study, the effects of root endophyte, Piriformospora indica strain DSM 11827 and nitrogen fixing bacterium, Azotobacter chroococcum strain W-5, either singly and/or in combination for artemisinin production in A. annua L. plants have been studied under poly house conditions. The plant growth was monitored by measuring parameters like height of plant, total dry weight and leaf yield with an increase of 63.51, 52.61 and 79.70% respectively, for treatment with dual biological consortium, as compared to that of control plants. This significant improvement in biomass was associated with higher total chlorophyll content (59.29%) and enhanced nutrition (especially nitrogen and phosphorus, 55.75 and 86.21% respectively). The concentration of artemisinin along with expression patterns of artemisinin biosynthesis genes were appreciably higher in dual treatment, which showed positive correlation. The study suggested the potential use of the consortium P. indica strain DSM 11827 and A. chroococcum strain W-5 in A. annua L. plants for increased overall productivity and sustainable agriculture.
目前，黄花蒿是青蒿素生产的主要来源。为控制疟疾的爆发，推荐使用青蒿素联合疗法（ACTs），因此ACTs的生产需要大量青蒿素以拯救数百万人的生命。这种抗疟药物在黄花蒿植物中的低产量（0.01 - 1.1%）导致其供应短缺和成本高昂，从而使其成为全球关注的焦点。在本研究中，在温室条件下研究了根内生真菌印度梨形孢菌株DSM 11827和固氮细菌圆褐固氮菌菌株W - 5单独和/或组合对黄花蒿植物青蒿素生产的影响。通过测量植株高度、总干重和叶片产量等参数来监测植物生长，与对照植株相比，双生物联合体处理的植株高度、总干重和叶片产量分别增加了63.51%、52.61%和79.70%。生物量的显著提高与总叶绿素含量的增加（59.29%）和营养的增强（特别是氮和磷，分别增加了55.75%和86.21%）有关。双处理中青蒿素的浓度以及青蒿素生物合成基因的表达模式明显更高，且呈正相关。该研究表明印度梨形孢菌株DSM 118,27和圆褐固氮菌菌株W - 5的联合体在黄花蒿植物中具有提高整体生产力和可持续农业的潜在用途。
