Quenon Camille, Hennebelle Thierry, Butaud Jean-François, Ho Raimana, Samaillie Jennifer, Neut Christel, Lehartel Tamatoa, Rivière Céline, Siah Ali, Bonneau Natacha, Sahpaz Sevser, Anthérieu Sébastien, Lebegue Nicolas, Raharivelomanana Phila, Roumy Vincent
UMRT 1158 BioEcoAgro, Métabolites spécialisés d'origine végétale, Université de Lille, JUNIA, Université de Liège, Université de Picardie Jules Verne, 59006 Lille, France.
Consultant in Forestry and Polynesian Botany, BP 52832, 98716 Pirae, Tahiti, French Polynesia.
Life (Basel). 2022 May 14;12(5):733. doi: 10.3390/life12050733.
A preliminary ethnopharmacological survey, achieved in French Polynesia, led to the collection of the most cited plants among 63 species used to treat “infectious” diseases, with a description of their medicinal uses. Bibliographical investigations and antimicrobial screening permitted the selection of the botanical species Syzygium malaccense (Myrtaceae) for phytochemical analysis. Leaves of Syzygium malaccense were usually used in mixture with rhizomes of Curcuma longa to treat infectious diseases such as cystitis. The methanolic plant extracts were tested in vitro with an agar microdilution method on 33 bacteria strains and 1 yeast to obtain their Minimal Inhibitory Concentration (MIC), and cytotoxicity against HepG2 cells were evaluated. Antimicrobial synergistic effects of methanolic plant extracts from leaves of Syzygium malaccense and rhizomes from Curcuma longa were also evaluated. The bio-guided isolation of leaf extract from Syzygium malaccense led to the identification of seven alkyl-salicylic acids (anacardic acids or ginkgolic acids C15:0, C15:1, C17:0, C17:1, C17:2, C17:3 and C19:1) described for the first time in this species. All compounds were tested against Staphylococcus aureus (18.75 < MIC < 75.0 µg/mL), Streptococcus pyogenes (2.34 < MIC < 18.75 µg/mL) and Pseudomonas aeruginosa (MIC = 150 µg/mL), and their structure−activity relationships were discussed. The methanolic extract and salicylic derivatives from S. malaccense showed an interesting antimicrobial activity against Gram+ bacteria, without toxicity on hepG2 cells at 400 μg/mL. Moreover, these antibacterial compounds have already been studied for their anti-inflammatory activity, which supports the therapeutic interest of S. malaccense against infectious diseases.
在法属波利尼西亚进行的一项初步民族药理学调查，收集了63种用于治疗“传染性”疾病的植物中被提及最多的植物，并对其药用用途进行了描述。文献研究和抗菌筛选使得选择了马六甲蒲桃（桃金娘科）进行植物化学分析。马六甲蒲桃的叶子通常与姜黄的根茎混合使用，以治疗膀胱炎等传染病。采用琼脂微量稀释法对该植物的甲醇提取物进行体外测试，以检测其对33种细菌菌株和1种酵母的最低抑菌浓度（MIC），并评估其对肝癌细胞HepG2的细胞毒性。还评估了马六甲蒲桃叶子和姜黄根茎的甲醇提取物的抗菌协同作用。对马六甲蒲桃叶子提取物进行生物导向分离，首次鉴定出7种烷基水杨酸（腰果酚酸或银杏酸C15:0、C15:1、C17:0、C17:1、C17:2、C17:3和C19:1）。所有化合物均针对金黄色葡萄球菌（18.75＜MIC＜75.0μg/mL）、化脓性链球菌（2.34＜MIC＜18.75μg/mL）和铜绿假单胞菌（MIC = 150μg/mL）进行了测试，并讨论了它们的构效关系。马六甲蒲桃的甲醇提取物和水杨酸衍生物对革兰氏阳性菌显示出有趣的抗菌活性，在400μg/mL时对肝癌细胞HepG2无毒性。此外，这些抗菌化合物的抗炎活性已经得到研究，这支持了马六甲蒲桃对传染病的治疗价值。
