Tu Youying, Kang Hyunuk, Kim Eunhye, Yang Jiangfan, He Puming, Wu Yuanyuan, Li Bo, Liu Xiaobo, Liu Junsheng
Department of Tea Science, Zhejiang University, Hangzhou 310058, China.
College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Metabolites. 2022 May 22;12(5):466. doi: 10.3390/metabo12050466.
Previously, we found that three types of Tieguanyin tea (Tgy-Q, Tgy-N and Tgy-C) extracts could alleviate Alzheimer's disease (AD) in a mouse model among which Tgy-C was more effective. In this study, APP/PS1 transgenic mice were used to investigate the metabolomic changes in the feces of mice treated with Tieguanyin tea extracts. Results showed that the profile of fecal metabolites was obviously changed in AD mice. Metabolomics analysis found the effects of Tgy-C, especially its decreasing effect on the fecal metabolites in AD mice-132 of the 155 differential metabolites were decreased. KEGG enrichment revealed that differential metabolites could participate in functional pathways including protein digestion and absorption, biosynthesis of amino acids and ABC transporters. Further comparisons of the metabolites between groups showed that although Tgy-N and Tgy-Q exerted a decreasing effect on the fecal metabolites, Tgy-C was more effective. Moreover, correlation analysis found that the levels of the fecal metabolites were highly correlated with the contents of functional components in tea extracts. Finally, 16S rDNA sequencing presented that Tieguanyin extracts modified the gut microbiota by targeting diverse bacteria. In this study, we investigated the differences of three types of Tieguanyin tea extracts on the fecal metabolites as well as the bacterial community of the gut microbiota in AD mice. The identified differential metabolites and the changed intestinal bacteria might provide potential diagnostic biomarkers for the occurrence and progression of AD.
此前，我们发现三种铁观音茶（Tgy-Q、Tgy-N和Tgy-C）提取物可缓解阿尔茨海默病（AD）小鼠模型中的病情，其中Tgy-C的效果更佳。在本研究中，利用APP/PS1转基因小鼠来研究经铁观音茶提取物处理的小鼠粪便中的代谢组学变化。结果显示，AD小鼠粪便代谢物谱明显改变。代谢组学分析发现了Tgy-C的作用效果，尤其是其对AD小鼠粪便代谢物的降低作用——155种差异代谢物中有132种减少。KEGG富集分析表明，差异代谢物可参与包括蛋白质消化与吸收、氨基酸生物合成以及ABC转运蛋白等功能途径。组间代谢物的进一步比较表明，虽然Tgy-N和Tgy-Q对粪便代谢物也有降低作用，但Tgy-C的效果更佳。此外，相关性分析发现，粪便代谢物水平与茶提取物中功能成分的含量高度相关。最后，16S rDNA测序表明，铁观音提取物通过靶向多种细菌改变了肠道微生物群。在本研究中，我们研究了三种铁观音茶提取物对AD小鼠粪便代谢物以及肠道微生物群细菌群落的影响。所鉴定出的差异代谢物和变化的肠道细菌可能为AD的发生和发展提供潜在的诊断生物标志物。
