Klose Corinna, Scuda Nelly, Ziegler Tobias, Eisenberger David, Hanczaruk Matthias, Riehm Julia M
Bavarian Health and Food Safety Authority, Eggenreuther Weg 43, 91058 Erlangen, Germany.
Bavarian Health and Food Safety Authority, Veterinaerstrasse 2, 85764 Oberschleißheim, Germany.
Microorganisms. 2022 Apr 23;10(5):885. doi: 10.3390/microorganisms10050885.
Worldwide, Dublin ( Dublin) is responsible for clinical disease in cattle and also in humans. In Southern Bavaria, Germany, the serovar was identified as a causative agent for 54 animal disease outbreaks in herds between 2017 and 2021. Most of these emerged from cattle herds ( = 50). Two occurred in pig farms and two in bovine herds other than cattle. Genomic analysis of 88 Dublin strains isolated during these animal disease outbreaks revealed 7 clusters with 3 different MLST-based sequence types and 16 subordinate cgMLST-based complex types. Antimicrobial susceptibility investigation revealed one resistant and three intermediate strains. Furthermore, only a few genes coding for bacterial virulence were found among the isolates. Genome analysis enables pathogen identification and antimicrobial susceptibility, serotyping, phylogeny, and follow-up traceback analysis. Mountain pastures turned out to be the most likely locations for transmission between cattle of different herd origins, as indicated by epidemiological data and genomic traceback analyses. In this context, Dublin shedding was also detected in asymptomatic herding dogs. Due to the high prevalence of Dublin in Upper Bavaria over the years, we suggest referring to this administrative region as "endemic". Consequently, cattle should be screened for salmonellosis before and after mountain pasturing.
在全球范围内，都柏林沙门氏菌（Dublin）可引发牛群以及人类的临床疾病。在德国巴伐利亚州南部，该血清型在2017年至2021年间被确定为54起动物疾病暴发的病原体，其中大部分暴发于牛群（50起），两起发生在养猪场，两起发生在除牛以外的其他牛群中。对在这些动物疾病暴发期间分离出的88株都柏林沙门氏菌菌株进行基因组分析，发现了7个簇，包含3种基于多位点序列分型（MLST）的不同序列类型和16种基于核心基因组多位点序列分型（cgMLST）的从属复合类型。抗菌药敏调查发现1株耐药菌株和3株中介菌株。此外，在分离株中仅发现少数编码细菌毒力的基因。基因组分析有助于病原体鉴定、抗菌药敏分析、血清分型、系统发育分析以及后续的溯源分析。流行病学数据和基因组溯源分析表明，山地牧场是不同牛群来源的牛之间最有可能的传播地点。在此背景下，还在无症状的放牧犬中检测到都柏林沙门氏菌的排菌情况。鉴于多年来上巴伐利亚州都柏林沙门氏菌的高流行率，我们建议将该行政区称为“地方性流行区”。因此，牛在山地放牧前后都应进行沙门氏菌病筛查。
