Kitae Hiroaki, Takagi Tomohisa, Naito Yuji, Inoue Ryo, Azuma Yuka, Torii Takashi, Mizushima Katsura, Doi Toshifumi, Inoue Ken, Dohi Osamu, Yoshida Naohisa, Kamada Kazuhiro, Uchiyama Kazuhiko, Ishikawa Takeshi, Konishi Hideyuki, Itoh Yoshito
Department of Molecular Gastroenterology and Hepatology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, 465 Kawaramachi Hirokoji, Kamigyo-ku, Kyoto 602-8566, Japan.
Department for Medical Innovation and Translational Medical Science, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan.
Microorganisms. 2022 May 18;10(5):1044. doi: 10.3390/microorganisms10051044.
The microbiota associated with relapse in patients with quiescent ulcerative colitis (qUC) remains unclear. Our objective was to analyze the fecal microbiota of Japanese patients with qUC and identify the relapse-associated microbiota. In this study, 59 patients with qUC and 59 healthy controls (HCs) were enrolled (UMIN 000019486), and their fecal microbiota was compared using 16S rRNA gene amplicon sequencing. We followed their clinical course up to 3.5 years and analyzed the relapse-associated microbiota. Potential functional changes in the fecal microbiota were evaluated using PICRUSt software and the Kyoto Encyclopedia of Genes and Genomes database. There were significant differences in fecal microbiota diversity between HC and qUC subjects, with 13 taxa characterizing each subject. Despite no significant difference in variation of microbiota in a single sample (α diversity) between patients in sustained remission and relapsed patients, the variation in microbial communities between samples (β diversity) was significantly different. was more abundant in the sustained remission patients, whereas and were more abundant in the relapsed patients. We clustered the entire cohort into four clusters, and Kaplan-Meier analysis revealed the subsequent clinical course of each cluster was different. We identified 48 metabolic pathways associated with each cluster using linear discriminant analysis effect size. We confirmed the difference in microbiota between patients with qUC and HCs and identified three genera associated with relapse. We found that the clusters based on these genera had different subsequent clinical courses and activated different metabolic pathways.
与静止期溃疡性结肠炎（qUC）患者复发相关的微生物群仍不清楚。我们的目标是分析日本qUC患者的粪便微生物群，并确定与复发相关的微生物群。在本研究中，纳入了59例qUC患者和59名健康对照（HCs）（UMIN 000019486），并使用16S rRNA基因扩增子测序比较了他们的粪便微生物群。我们对他们长达3.5年的临床病程进行了随访，并分析了与复发相关的微生物群。使用PICRUSt软件和京都基因与基因组百科全书数据库评估粪便微生物群的潜在功能变化。HC和qUC受试者之间的粪便微生物群多样性存在显著差异，每个受试者有13个分类群特征。尽管处于持续缓解期的患者和复发患者之间单个样本中的微生物群变异（α多样性）没有显著差异，但样本之间微生物群落的变异（β多样性）有显著差异。在持续缓解期患者中更为丰富，而在复发患者中更为丰富。我们将整个队列聚类为四个簇，Kaplan-Meier分析显示每个簇的后续临床病程不同。我们使用线性判别分析效应大小确定了与每个簇相关的48条代谢途径。我们证实了qUC患者和HCs之间微生物群的差异，并确定了三个与复发相关的属。我们发现基于这些属的簇具有不同的后续临床病程并激活了不同的代谢途径。
