National Institute of Genetic Engineering and Biotechnology (NIGEB), Shahrak-e Pajoohesh km 15, Tehran-Karaj Highway, Tehran P.O. Box 14965/161, Iran.
Department of Chemical Engineering, University of Johannesburg, Johannesburg 2028, South Africa.
Molecules. 2022 May 23;27(10):3336. doi: 10.3390/molecules27103336.
Cyanide is a poisonous and dangerous chemical that binds to metals in metalloenzymes, especially cytochrome C oxidase and, thus, interferes with their functionalities. Different pathways and enzymes are involved during cyanide biodegradation, and cyanide hydratase is one of the enzymes that is involved in such a process. In this study, cyanide resistance and cyanide degradation were studied using 24 fungal strains in order to find the strain with the best capacity for cyanide bioremediation. To confirm the capacity of the tested strains, cyano-bioremediation and the presence of the gene that is responsible for the cyanide detoxification was assessed. From the tested organisms, () had a significant capability to resist and degrade cyanide at a 15 mM concentration, where it achieved an efficiency of 75% in 7 days. The gene network analysis of enzymes that are involved in cyanide degradation revealed the involvement of cyanide hydratase, dipeptidase, carbon-nitrogen hydrolase-like protein, and ATP adenylyltransferase. This study revealed that was more efficient in degrading cyanide than the other tested fungal organisms, and molecular analysis confirmed the experimental observations.
氰化物是一种有毒危险的化学物质，它与金属酶中的金属结合，特别是细胞色素 C 氧化酶，从而干扰其功能。氰化物生物降解过程涉及不同的途径和酶，氰化物水解酶就是参与这一过程的酶之一。本研究使用 24 株真菌菌株研究了氰化物的抗性和降解，以寻找具有最佳氰化物生物修复能力的菌株。为了确认测试菌株的能力，评估了氰基生物修复和负责氰化物解毒的基因的存在。在所测试的生物中，（）在 15mM 浓度下具有显著的抵抗和降解氰化物的能力，在 7 天内达到 75%的效率。参与氰化物降解的酶的基因网络分析表明，氰化物水解酶、二肽酶、碳氮水解酶样蛋白和 ATP 腺苷酰转移酶的参与。这项研究表明，与其他测试的真菌生物相比，（）在降解氰化物方面更有效，分子分析证实了实验观察结果。
