Khramtsov Pavel, Kropaneva Maria, Minin Artem, Bochkova Maria, Timganova Valeria, Maximov Andrey, Puzik Alexey, Zamorina Svetlana, Rayev Mikhail
Faculty of Biology, Perm State University, 614068 Perm, Russia.
Lab of Ecological Immunology, Institute of Ecology and Genetics of Microorganisms, 614081 Perm, Russia.
Nanomaterials (Basel). 2022 May 10;12(10):1630. doi: 10.3390/nano12101630.
Prussian blue nanozymes possessing peroxidase-like activity gather significant attention as alternatives to natural enzymes in therapy, biosensing, and environmental remediation. Recently, Prussian blue nanoparticles with enhanced catalytic activity prepared by reduction of FeCl/K[Fe(CN)] mixture have been reported. These nanoparticles were denoted as 'artificial peroxidase' nanozymes. Our study provides insights into the process of their synthesis. We studied how the size of nanozymes and synthesis yield can be controlled via adjustment of the synthesis conditions. Based on these results, we developed a reproducible and scalable method for the preparation of 'artificial peroxidase' with tunable sizes and enhanced catalytic activity. Nanozymes modified with gelatin shell and functionalized with affine molecules were applied as labels in colorimetric immunoassays of prostate-specific antigen and tetanus antibodies, enabling detection of these analytes in the range of clinically relevant concentrations. Protein coating provides excellent colloidal stability of nanozymes in physiological conditions and stability upon long-term storage.
具有类过氧化物酶活性的普鲁士蓝纳米酶作为天然酶在治疗、生物传感和环境修复方面的替代品受到了广泛关注。最近，有报道称通过还原FeCl/K[Fe(CN)]混合物制备出了具有增强催化活性的普鲁士蓝纳米颗粒。这些纳米颗粒被称为“人工过氧化物酶”纳米酶。我们的研究深入探讨了它们的合成过程。我们研究了如何通过调整合成条件来控制纳米酶的尺寸和合成产率。基于这些结果，我们开发了一种可重现且可扩展的方法，用于制备具有可调尺寸和增强催化活性的“人工过氧化物酶”。用明胶壳修饰并用亲和分子功能化的纳米酶被用作前列腺特异性抗原和破伤风抗体比色免疫分析中的标记物，能够在临床相关浓度范围内检测这些分析物。蛋白质涂层在生理条件下为纳米酶提供了出色的胶体稳定性以及长期储存时的稳定性。
