Krumova Stefka, Andonova Ivona, Stefanova Radostina, Miteva Polina, Nenkova Galina, Hübschen Judith M
National Reference Laboratory "Measles, Mumps, Rubella", Department of Virology, National Center of Infectious and Parasitic Diseases, 1233 Sofia, Bulgaria.
Specialized Hospital for Active Treatment of Children's Diseases "Prof. Dr. Ivan Mitev" EAD, 1612 Sofia, Bulgaria.
Pathogens. 2022 Apr 21;11(5):497. doi: 10.3390/pathogens11050497.
Primate erythroparvovirus 1, commonly referred to as Parvovirus B19 (B19V), is a DNA virus that normally results in a mild childhood infection called "erythema infectiosum". Besides respiratory spread, B19V can also be transmitted through transfusions, which may result in persistent anemia in immunodeficient hosts. Dialysis patients often face acute or chronic anemia after infection with B19V. Here, we describe the laboratory investigation of 21 patients with hematological disorders for B19V infections. B19V DNA was detected in 13 (62%) of them, with specific IgM antibodies in three of the DNA positives. All 13 patients received treatment and were laboratory-monitored over a period of one year. In only two patients (a 14-year-old child with a kidney transplantation and a 39-year-old patient with aplastic anemia), markers of recent B19V infection were still detectable in follow-up samples. For four B19V DNA positive samples, short sequences could be obtained, which clustered with genotype 1a reference strains. Our findings suggest that all cases of hematological disorders should be examined for specific B19V antibodies and DNA for accurate diagnosis and appropriate patient management.
灵长类细小病毒1，通常称为细小病毒B19（B19V），是一种DNA病毒，通常会导致一种称为“传染性红斑”的轻度儿童感染。除了通过呼吸道传播外，B19V还可通过输血传播，这可能导致免疫缺陷宿主出现持续性贫血。透析患者感染B19V后常面临急性或慢性贫血。在此，我们描述了对21例血液系统疾病患者进行B19V感染的实验室调查。其中13例（62%）检测到B19V DNA，DNA阳性的3例中有特异性IgM抗体。所有13例患者均接受了治疗，并在一年时间内进行了实验室监测。仅在2例患者（1例14岁肾移植儿童和1例39岁再生障碍性贫血患者）的随访样本中仍可检测到近期B19V感染的标志物。对于4份B19V DNA阳性样本，可获得短序列，这些序列与1a基因型参考菌株聚类。我们的研究结果表明，所有血液系统疾病病例均应检测特异性B19V抗体和DNA，以进行准确诊断和适当的患者管理。
