Faculty of Food Engineering, Stefan cel Mare University of Suceava, 720229 Suceava, Romania.
Nutrients. 2022 May 20;14(10):2142. doi: 10.3390/nu14102142.
The aim of this study was to develop films based on β-glucans in association with pomegranate juice for its potential in metabolic disorders such as diabetes due to plenty of bioactive compounds from the film composition. Initially, a Box-Behnken design was generated by varying the level of β-glucan content (0.5, 1, 1.5 g), sodium alginate (0.2, 0.4, 0.6 g) and pomegranate juice (10, 20, 30 mL) for development of films. Subsequently, glycerin was added as 25% of the total dry matter. The optimization of the films prepared by the solvent casting method was conducted based on the different responses such as: water vapor transmission rate (WVTR), water vapor permeability (WVP), thickness, density, moisture content, solubility, film opacity and color. The water activity profile and FT-IR analysis were performed in all tests. The model was used to determine the optimal experimental values considering that the optimal film will make a sustained contribution to diabetes. The optimal values of the film sample made of β-glucans, sodium alginate, pomegranate juice and glycerin make it befitting for packaging dry powdered pharmaceuticals. Finally, antimicrobial activity against Gram-negative and Gram-positive bacteria, UV barrier properties and microcrack and pore detections through SEM were also investigated for the optimal film sample.
本研究旨在开发基于β-葡聚糖的薄膜，结合石榴汁，因为其薄膜组成物中有大量的生物活性化合物，具有治疗糖尿病等代谢紊乱的潜力。首先，通过改变β-葡聚糖含量（0.5、1、1.5 g）、海藻酸钠（0.2、0.4、0.6 g）和石榴汁（10、20、30 mL）水平，生成了 Box-Behnken 设计，以开发薄膜。随后，以总干物质的 25%添加甘油。基于水蒸气透过率（WVTR）、水蒸气透过率（WVP）、厚度、密度、水分含量、溶解度、薄膜不透明度和颜色等不同响应，对溶剂浇铸法制备的薄膜进行了优化。在所有测试中均进行了水活度分析和 FT-IR 分析。该模型用于确定最佳实验值，因为最佳薄膜将对糖尿病有持续的治疗作用。由β-葡聚糖、海藻酸钠、石榴汁和甘油制成的薄膜样品的最佳值适合包装干粉状药物。最后，还对最佳薄膜样品进行了针对革兰氏阴性菌和革兰氏阳性菌的抗菌活性、UV 阻隔性能以及 SEM 下的微裂纹和孔隙检测。
