Fonseca Jose P, Lakshmanan Venkatachalam, Boschiero Clarissa, Mysore Kirankumar S
The Noble Research Institute, Ardmore, OK 73401, USA.
Institute for Agricultural Biosciences, Oklahoma State University, Ardmore, OK 73401, USA.
Plants (Basel). 2022 May 17;11(10):1323. doi: 10.3390/plants11101323.
Pathogen associated molecular pattern (PAMP) triggered immunity (PTI) is the first line of plant defense. We hypothesized that the absence of pattern recognition receptors (PRRs) in plants could influence the rhizosphere microbiome. Here, we report sequencing of the 16S ribosomal RNA gene and the fungal ribosomal RNA internal transcribed spacer region of rhizosphere DNA from three PRR mutants involved in plant innate immunity (, , and ). We conducted experiments in a growth chamber using native soil from the Red River Farm (Terral, OK, USA) to detect microbial community shifts in the rhizosphere that may occur in the absence of PRR receptors compared to wild-type (WT; Col-0) plants. No difference in the α-diversity of the rhizosphere microbial population was observed between the PRR mutants tested and the WT. Plant host genotype had a significant impact in bacterial β-diversity only between the mutant and the WT. Surprisingly, no significant changes in fungal β-diversity were observed between the PRR mutants and WT, although we observed an increase in relative abundance for the cup fungi () in the mutant. This finding suggests that the FLS2 receptor can modulate the rhizosphere-associated microbiome β-diversity and expands the list of current known genotypes that can modulate the rhizosphere microbiota.
病原体相关分子模式（PAMP）触发的免疫（PTI）是植物防御的第一道防线。我们假设植物中模式识别受体（PRR）的缺失可能会影响根际微生物群。在此，我们报告了来自参与植物先天免疫的三个PRR突变体（ 、 和 ）的根际DNA的16S核糖体RNA基因和真菌核糖体RNA内部转录间隔区的测序结果。我们在美国俄克拉荷马州特拉尔红河农场使用原生土壤在生长室中进行实验，以检测与野生型（WT；Col-0）植物相比，在没有PRR受体的情况下根际可能发生的微生物群落变化。在所测试的PRR突变体和WT之间，未观察到根际微生物种群的α多样性有差异。仅在 突变体和WT之间，植物宿主基因型对细菌β多样性有显著影响。令人惊讶的是，尽管我们观察到 突变体中杯状真菌（ ）的相对丰度增加，但在PRR突变体和WT之间未观察到真菌β多样性的显著变化。这一发现表明，FLS2受体可以调节根际相关微生物群的β多样性，并扩展了当前已知可调节根际微生物群的基因型列表。
