Cahyaningsih Ria, Compton Lindsey Jane, Rahayu Sri, Magos Brehm Joana, Maxted Nigel
School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK.
Research Center for Plant Conservation, Botanical Gardens and Forestry, National Research and Innovation Agency, Bogor 16122, Indonesia.
Plants (Basel). 2022 May 21;11(10):1375. doi: 10.3390/plants11101375.
Over the past decade, plant DNA barcoding has emerged as a scientific breakthrough and is often used to help with species identification or as a taxonomical tool. DNA barcoding is very important in medicinal plant use, not only for identification purposes but also for the authentication of medicinal products. Here, a total of 61 Indonesian medicinal plant species from 30 families and a pair of ITS2, , , and primers were used for a DNA barcoding study consisting of molecular and sequence analyses. This study aimed to analyze how the four identified DNA barcoding regions (ITS2, , , and ) aid identification and conservation and to investigate their effectiveness for DNA barcoding for the studied species. This study resulted in 212 DNA barcoding sequences and identified new ones for the studied medicinal plant species. Though there is no ideal or perfect region for DNA barcoding of the target species, we recommend as the main region for Indonesian medicinal plant identification, with ITS2 and as alternative or complementary regions. These findings will be useful for forensic studies that support the conservation of medicinal plants and their national and global use.
在过去十年中，植物DNA条形码技术已成为一项科学突破，常被用于帮助物种鉴定或作为一种分类学工具。DNA条形码技术在药用植物应用中非常重要，不仅用于鉴定目的，还用于药品的认证。在此，来自30个科的总共61种印度尼西亚药用植物物种以及一对ITS2、 、 、 和引物被用于一项由分子和序列分析组成的DNA条形码研究。本研究旨在分析四个已鉴定的DNA条形码区域（ITS2、 、 、 和 ）如何辅助鉴定和保护，并研究它们对所研究物种进行DNA条形码分析的有效性。本研究产生了212个DNA条形码序列，并为所研究的药用植物物种鉴定出了新的序列。尽管对于目标物种的DNA条形码分析没有理想或完美的区域，但我们建议 将 作为印度尼西亚药用植物鉴定的主要区域，ITS2和 作为替代或互补区域。这些发现将有助于支持药用植物保护及其国内和全球利用的法医研究。
