Vodicka Juraj, Wikarska Monika, Trudicova Monika, Juglova Zuzana, Pospisilova Aneta, Kalina Michal, Slaninova Eva, Obruca Stanislav, Sedlacek Petr
Faculty of Chemistry, Brno University of Technology, Purkynova 118, 612 00 Brno, Czech Republic.
Polymers (Basel). 2022 May 13;14(10):1990. doi: 10.3390/polym14101990.
A novel model of biodegradable PHA copolymer films preparation was applied to evaluate the biodegradability of various PHA copolymers and to discuss its biomedical applicability. In this study, we illustrate the potential biomaterial degradation rate affectability by manipulation of monomer composition via controlling the biosynthetic strategies. Within the experimental investigation, we have prepared two different copolymers of 3-hydroxybutyrate and 4-hydroxybutyrate-P(3HB--36 mol.% 4HB) and P(3HB--66 mol.% 4HB), by cultivating the thermophilic bacterial strain sp. H1 and further investigated its degradability in simulated body fluids (SBFs). Both copolymers revealed faster weight reduction in synthetic gastric juice (SGJ) and artificial colonic fluid (ACF) than simple homopolymer P3HB. In addition, degradation mechanisms differed across tested polymers, according to SEM micrographs. While incubated in SGJ, samples were fragmented due to fast hydrolysis sourcing from substantially low pH, which suggest abiotic degradation as the major degradation mechanism. On the contrary, ACF incubation indicated obvious enzymatic hydrolysis. Further, no cytotoxicity of the waste fluids was observed on CaCO-2 cell line. Based on these results in combination with high production flexibility, we suggest P(3HB--4HB) copolymers produced by sp. H1 as being very auspicious polymers for intestinal in vivo treatments.
一种新型的可生物降解PHA共聚物薄膜制备模型被用于评估各种PHA共聚物的生物降解性，并探讨其在生物医学方面的适用性。在本研究中，我们通过控制生物合成策略来操纵单体组成，从而阐述了潜在生物材料降解速率的可影响性。在实验研究中，我们通过培养嗜热细菌菌株sp. H1制备了两种不同的3-羟基丁酸酯和4-羟基丁酸酯的共聚物——P(3HB-36 mol.% 4HB)和P(3HB-66 mol.% 4HB)，并进一步研究了它们在模拟体液（SBF）中的降解性。与简单的均聚物P3HB相比，这两种共聚物在合成胃液（SGJ）和人工结肠液（ACF）中均显示出更快的重量减轻。此外，根据扫描电子显微镜（SEM）照片，不同测试聚合物的降解机制有所不同。在SGJ中孵育时，由于pH值极低导致快速水解，样品发生破碎，这表明非生物降解是主要的降解机制。相反，ACF孵育表明存在明显的酶促水解。此外，在CaCO-2细胞系上未观察到废液的细胞毒性。基于这些结果以及高生产灵活性，我们认为由sp. H1产生的P(3HB-4HB)共聚物是用于肠道体内治疗的非常有前景的聚合物。
