大鼠坐骨神经挤压伤模型的评价方法。
Evaluation methods of a rat sciatic nerve crush injury model.
机构信息
Department of Tuina, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, 200437 Shanghai, China.
Acupuncture and Tuina College, Shanghai University of Traditional Chinese Medicine, 201023 Shanghai, China.
出版信息
J Integr Neurosci. 2022 May 11;21(3):91. doi: 10.31083/j.jin2103091.
BACKGROUND
The rat sciatic nerve crush injury model is one of the most commonly used models to research peripheral nerve injury (PNI), however, the evaluation of the model preparation lacks exact standards. This study aimed to investigate accurately assessment methods for research concerning the rat sciatic nerve crush injury.
METHODS
The sciatic nerve crush injury model of was performed using the FST toothless forceps. The corresponding locations and pressures of different ratchet strengths were assessed by using CMAP, behavioral, and morphological methods.
RESULTS
In each group of PNI, motor and sensory functions were gradually restricted on the injured side of rats as the applied pressure increased. CMAP was more sensitive to nerve injury arising out of the force values obtained from the forceps.
CONCLUSIONS
As a sensitive indicator for PNI, the neuroelectrophysiological examination was more likely to reflect the morphological changes of injured nerves. These findings may provide a standardized approach to sciatic crush injury modelling.
背景
大鼠坐骨神经挤压伤模型是研究周围神经损伤（PNI）最常用的模型之一，但模型制备的评估缺乏确切的标准。本研究旨在探讨大鼠坐骨神经挤压伤研究的准确评估方法。
方法
使用 FST 无齿镊子进行坐骨神经挤压伤模型。通过 CMAP、行为和形态学方法评估不同棘轮强度的相应位置和压力。
结果
在每组 PNI 中，随着施加压力的增加，大鼠受伤侧的运动和感觉功能逐渐受限。CMAP 比从镊子获得的力值更能灵敏地反映出神经损伤。
结论
作为 PNI 的敏感指标，神经电生理检查更有可能反映损伤神经的形态变化。这些发现可能为坐骨挤压伤建模提供一种标准化方法。
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