Pedersen Kasper, Blirup-Plum Sophie Amalie, Kristensen Charlotte Sonne, Kvisgaard Lise Kirstine, Skade Lotte, Jensen Henrik Elvang, Larsen Lars Erik
SEGES Danish Pig Research Centre, Aarhus, Denmark.
Department of Veterinary and Animal Sciences, University of Copenhagen, Frederiksberg, Denmark.
Front Microbiol. 2022 May 11;13:874498. doi: 10.3389/fmicb.2022.874498. eCollection 2022.
Major geographical transmission of porcine reproductive and respiratory syndrome virus (PRRSV) occurs semen when a boar stud is infected. This happened in Denmark in 2019, providing an opportunity to compare previous experimental PRRSV boar studies with natural PRRSV-1 infection in boars. The aim of this study was to investigate the association between the presence of PRRSV RNA in serum, semen, testicles, and epididymis of boars naturally infected with PRRSV and to describe the histological lesions in the testes and epididymis combined with direct visualisation of PRRSV-infected cells by immunohistochemical staining (IHC). The exact timing of infection of each boar was not determined, but based on serology the boars were divided into two groups: acute and late infections. All boars included were sampled the same day. In this study, 35 boars and 10 healthy boars from another PRRSV-negative boar stud were included as histological controls. PRRSV RNA was found most often in serum (51%) and least frequently in semen (22%) and was more often detected in the reproductive tract in the acute phase of infection ( < 0.0001; RR: 2.58). Mononuclear cells and multinuclear giant cells were present in the adluminal compartment of the testis and epididymis in PRRSV-infected boars, but not in control boars ( < 0.05), which supports the hypothesis that macrophages are involved in the venereal spread of the virus.
当种猪场感染猪繁殖与呼吸综合征病毒（PRRSV）时，PRRSV会发生主要的地理传播。2019年丹麦就出现了这种情况，这为将之前关于PRRSV的公猪实验研究与公猪自然感染PRRSV-1的情况进行比较提供了机会。本研究的目的是调查自然感染PRRSV的公猪血清、精液、睾丸和附睾中PRRSV RNA的存在情况之间的关联，并结合免疫组织化学染色（IHC）直接观察PRRSV感染细胞，描述睾丸和附睾的组织学病变。每头公猪的确切感染时间未确定，但根据血清学将公猪分为两组：急性感染组和晚期感染组。所有纳入的公猪均在同一天采样。在本研究中，纳入了35头公猪和来自另一个PRRSV阴性种猪场的10头健康公猪作为组织学对照。PRRSV RNA最常在血清中被发现（51%），在精液中最少见（22%），并且在感染急性期的生殖道中更常被检测到（<0.0001；相对风险：2.58）。PRRSV感染的公猪睾丸和附睾的管腔隔中有单核细胞和多核巨细胞，但对照公猪中没有（<0.05），这支持了巨噬细胞参与病毒性传播的假说。
