Movahedpour Ahmad, Mostafavi-Pour Zohreh, Sarkari Bahador, Taheri-Anganeh Mortaza, Nezafat Navid, Savardashtaki Amir, Ghasemi Younes
Department of Medical Biotechnology, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences Shiraz, Iran.
Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran.
Avicenna J Med Biotechnol. 2022 Apr-Jun;14(2):114-124. doi: 10.18502/ajmb.v14i2.8886.
Serological diagnosis of () is fre-quently challenging because of cross-reactivity with other parasitic nematodes. Therefore, it is necessary to introduce novel serological tests with high performance to properly diagnose this neglected parasitic infection. The purpose of the current study was to design a multi-epitope construct for the diagnosis of .
For the purpose of this study, first, highly antigenic segments and potential immunodominant epitopes of were identified from two antigenic proteins, and then all of the selected parts were linked by an appropriate linker. Next, the physicochemical features of the designed construct were analyzed. Then, tertiary structures of the construct were built and evaluated to find out the best one. Lastly, the amino acid sequence was reverse-translated and optimized for over-expression in (.
The bioinformatic evaluation indicated that the designed protein construct could be hydrophilic, thermostable, and acidic and the estimated half-life was more than 10 in .
According to the results of the study, the designed construct could be used as an efficient antigen in the ELISA system for serological diagnosis of human strong-yloidiasis.
由于与其他寄生线虫存在交叉反应，（某种寄生虫，原文未明确）的血清学诊断常常具有挑战性。因此，有必要引入高性能的新型血清学检测方法，以准确诊断这种被忽视的寄生虫感染。本研究的目的是设计一种用于诊断（该寄生虫）的多表位构建体。
为实现本研究目的，首先从两种抗原蛋白中鉴定出（该寄生虫）的高抗原性片段和潜在免疫显性表位，然后用合适的连接子将所有选定部分连接起来。接下来，分析所设计构建体的理化特性。然后，构建并评估该构建体的三级结构，以找出最佳结构。最后，对氨基酸序列进行反向翻译并优化，以便在（某种表达系统，原文未明确）中进行过表达。
生物信息学评估表明，所设计的蛋白质构建体可能具有亲水性、热稳定性且呈酸性，在（某种环境，原文未明确）中的估计半衰期超过10（单位未明确）。
根据研究结果，所设计的构建体可作为酶联免疫吸附测定（ELISA）系统中用于人类类圆线虫病血清学诊断的有效抗原。
