采用伊红全脑铺片染色分析阿尔茨海默病果蝇模型中的神经退行性变。

Buck Institute for Research on Aging, Novato, CA 94945, USA.

University California San Francisco, San Francisco, CA 94158, USA.

STAR Protoc. 2022 May 25;3(2):101377. doi: 10.1016/j.xpro.2022.101377. eCollection 2022 Jun 17.

In the fly brain, neurodegeneration is detected by the presence of vacuoles using conventional hematoxylin and eosin (H&E) or phalloidin staining, which are lengthy and expensive processes. Here, we present a faster and cost-effective 2-day protocol to visualize vacuoles in a fly model of Alzheimer disease. We describe eosin staining in the whole-brain mount, followed by confocal microscopy and image analysis with an open source Fiji plugin. This protocol can be applied to visualize different modules in the fly brain.

在果蝇大脑中，通过使用常规的苏木精和伊红（H&E）或鬼笔环肽染色来检测空泡的存在，从而检测神经退行性变，但这些方法冗长且昂贵。在这里，我们提出了一种更快、更具成本效益的 2 天方案，用于可视化阿尔茨海默病果蝇模型中的空泡。我们描述了全脑标本中的曙红染色，然后进行共聚焦显微镜检查和使用开源 Fiji 插件进行图像分析。该方案可用于可视化果蝇大脑中的不同模块。