提取物的毒性评估和伤口愈合活性研究。
Assessment of Toxicity and Wound Healing Activity of Extract.
机构信息
Pharmacology Division, CSIR-National Botanical Research Institute, Lucknow, India.
Amity Institute of Pharmacy, Amity University Uttar Pradesh, Lucknow, India.
出版信息
Drug Chem Toxicol. 2023 Nov;46(4):625-633. doi: 10.1080/01480545.2022.2075378. Epub 2022 May 29.
AIM
The aim of the study was to assess the toxicity profile of extract and evaluate its wound healing activity.
METHODS
wound healing activity of extract (5% and 10%) was performed using Clonogenic and Scratch assays. The toxicity profile of extract ointment was evaluated on animals using acute toxicity and dermal toxicity tests. wound healing activity of extract ointment (5% and 10%) was used to determine tensile strength in the incision wound healing model.
RESULTS
Results exhibited that the extract was safe up to 2000 mg/kg per oral dose and non-reactive while applied topically. results showed that extract closed the wound gap created by 97.13% in 48 h. The clonogenic assay revealed that the surviving factor for HaCaT cells and MEF cells was 0.78 and 0.85 after treated with 10% concentrations of . The tensile strength exhibited by 5% and 10% groups was 395.4 g and 558.5 g in comparison to the control group.
CONCLUSION
Thus, extract can be used as an alternative safe drug therapy against topical wounds.
目的
本研究旨在评估提取物的毒性概况，并评价其伤口愈合活性。
方法
采用集落形成和划痕实验评价提取物（5%和 10%）的伤口愈合活性。采用急性毒性和皮肤毒性试验评价提取物软膏的毒性概况。采用切口愈合模型评价提取物软膏（5%和 10%）的伤口愈合活性对拉伸强度的影响。
结果
结果表明，提取物的口服剂量高达 2000mg/kg 是安全的，且局部应用时无反应。结果显示，提取物在 48 小时内将伤口间隙闭合了 97.13%。集落形成实验表明，10%浓度的 处理后 HaCaT 细胞和 MEF 细胞的存活因子分别为 0.78 和 0.85。与对照组相比，5%和 10%组的拉伸强度分别为 395.4g 和 558.5g。
结论
因此，提取物可作为局部伤口的替代安全药物治疗方法。
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