低频超声和氮限制诱导斜生栅藻 FACHB-12 生物量和脂类积累的增强。
Low-frequency ultrasound and nitrogen limitation induced enhancement in biomass production and lipid accumulation of Tetradesmus obliquus FACHB-12.
机构信息
School of Resources, Environment and Materials, Guangxi University, Nanning, Guangxi 530004, PR China.
Guangxi Key Laboratory of Electrochemical Energy Materials, Institute of Optical Materials and Chemical Biology, School of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, PR China; MOE Key Laboratory of New Processing Technology for Nonferrous Metals and Materials, Guangxi Key Laboratory of Processing for Non-ferrous Metals and Featured Materials, Guangxi University, Nanning 530004, PR China.
出版信息
Bioresour Technol. 2022 Aug;358:127387. doi: 10.1016/j.biortech.2022.127387. Epub 2022 May 27.
The two-stage cultivation strategy was optimized in this study to simultaneously promote the growth and lipid accumulation of Tetradesmus obliquus. Results showed that the optimal dual-stress conditions were nitrogen concentration at 25 mg N·L and low-frequency ultrasound at 200 Watt, 1 min, and 8 h interval. The biomass and lipid content of Tetradesmus obliquus were increased by 32.1% and 44.5%, respectively, comparing to the control, and the lipid productivity reached 86.97 mg·L·d at the end of the cultivation period. The protein and photosynthetic pigment contents of microalgae decreased by 22.4% and 14.0% under dual stress comparing to the control environment. In addition, dual stress cultivation of microalgae presented higher level of antioxidant capacity to balance to oxidation level in microalgal cells. This study provides a new insight for microalgae growth and lipid accumulation with dual stress stimulation.
本研究采用两段式培养策略，同时促进斜生栅藻的生长和脂质积累。结果表明，最佳双重胁迫条件为氮浓度 25mgN·L 和低频超声 200 瓦、1 分钟、间隔 8 小时。与对照组相比，斜生栅藻的生物量和脂质含量分别增加了 32.1%和 44.5%，培养期末的脂质生产力达到 86.97mg·L·d。与对照环境相比，微藻在双重胁迫下的蛋白质和光合色素含量分别下降了 22.4%和 14.0%。此外，微藻的双重胁迫培养表现出更高的抗氧化能力来平衡微藻细胞中的氧化水平。本研究为双重胁迫刺激下微藻的生长和脂质积累提供了新的见解。
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