Department of Animal Medicine and Surgery, Universitat Autònoma de Barcelona, 08193, Barcelona, Spain.
Department of Cell Biology, Physiology and Immunology, Universitat Autònoma de Barcelona, 08193, Barcelona, Spain.
BMC Vet Res. 2022 May 30;18(1):206. doi: 10.1186/s12917-022-03317-1.
Beta adrenergic receptors (β-AR) play a key role in regulating several hallmark pathways of both benign and malignant human and canine tumors. There is scarce information on the expression of β-AR in canine vascular tumors. Therefore, the purpose of the present research work was to study the mRNA expression levels of the three subtypes of the β-AR genes (ADRB1, ADRB2, ADRB3) in hemangiosarcoma (HSA) and hemangioma (HA), as well as in vascular hamartomas (VH) from dogs.Fifty samples (n = 50) were obtained from 38 dogs. Twenty-three animals had HSA, eight animals HA and seven animals VH. HSA were auricular (n = 8), splenic (n = 5), cutaneous (n = 6), auricular and splenic (n = 2), cutaneous-muscular (n = 1) and disseminated (n = 1). There were seven cases of HSA that were divided into primary tumor and secondary (metastatic) tumor. Skin and muscle samples with a normal histological study were used as control group. ADRB gene expression was determinate in all samples by real-time quantitative PCR. Results showed that ADRB1, ADRB2 and ADRB3 were overexpressed in HSA when compared to the control group. ADRB2 was overexpressed in HA when compared to the control group. HSA express higher values of ADBR1 (p = 0.0178) compared to VH. There was a high inter-individual variability in the expression of the three subtypes of ADBR. No statistically significant difference in the expression of ADBR genes were observed between HSA primary when compared to metastatic or in different anatomical locations. In conclusion, canine HSA overexpress the three β-AR subtypes and canine HA β2-AR. High variability was observed in β-AR mRNA levels amongst HSA cases.
β肾上腺素能受体（β-AR）在调节人类和犬良性和恶性肿瘤的几个标志性途径中起着关键作用。关于犬血管肿瘤中β-AR 的表达信息很少。因此，本研究工作的目的是研究三种β-AR 基因亚型（ADRB1、ADRB2、ADRB3）在血管肉瘤（HSA）和血管瘤（HA）以及犬血管错构瘤（VH）中的 mRNA 表达水平。
从 38 只狗中获得了 50 个样本（n=50）。23 只动物患有 HSA，8 只动物患有 HA，7 只动物患有 VH。HSA 为耳（n=8）、脾（n=5）、皮肤（n=6）、耳脾（n=2）、皮肤肌肉（n=1）和弥散性（n=1）。有 7 例 HSA 分为原发性肿瘤和继发性（转移性）肿瘤。皮肤和肌肉样本组织学检查正常，作为对照组。所有样本均通过实时定量 PCR 测定 ADRB 基因表达。结果表明，与对照组相比，HSA 中 ADRB1、ADRB2 和 ADRB3 过度表达。与对照组相比，HA 中 ADRB2 过度表达。与 VH 相比，HSA 表达更高水平的 ADBR1（p=0.0178）。ADRB 三种亚型的表达存在个体间高度差异。HSA 原发性与转移性或不同解剖部位之间，ADRB 基因的表达无统计学差异。结论是，犬 HSA 过度表达三种β-AR 亚型和犬 HAβ2-AR。在 HSA 病例中，β-AR mRNA 水平存在高度变异性。
