Khateeb Sahar, Albalawi Aishah, Alkhedaide Adel
Biochemistry Division, Department of Chemistry, Faculty of Science, Fayoum University, Fayoum, Egypt.
Biology Department, Faculty of Science, University of Tabuk, Tabuk, Saudi Arabia.
Diabetes Metab Syndr Obes. 2022 May 24;15:1589-1596. doi: 10.2147/DMSO.S355677. eCollection 2022.
Obesity is a chronic metabolic disorder that results in excessive energy accumulated in adipose tissue causing dysfunction of adipocytes, inflammation, and oxidative stress. Diosgenin (DG), a steroidal saponin produced by several plants, has been reported to have antioxidant activity. This study aimed to evaluate the effects of diosgenin on oxidative stress and inflammation in mice fed with a high-fat diet (HFD).
Thirty adult male mice were divided into three groups including the control group, mice fed with a normal diet; the HFD group, mice fed with a high-fat diet for 6 weeks; and the HFD+DG group, mice fed with a high-fat diet and diosgenin daily for 6 weeks. Interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α), malondialdehyde (MDA), and total antioxidant capacity (TAC) activities were evaluated. Histopathological changes in the adipose tissues have been investigated.
Data showed that diosgenin increased TAC activities with a concomitant decrease in MDA levels. As well, DG reduces the TNF and IL-6 levels. The histopathological changes in the adipose tissues due to high-fat consumption were restored upon DG supplementation.
Our results suggested that diosgenin is a promising agent for regulating obesity by increasing the levels of antioxidants, modifying oxidative stress and pro-inflammatory cytokines, which might prevent the onset of many diseases.
肥胖是一种慢性代谢紊乱疾病，会导致能量在脂肪组织中过度积累，从而引起脂肪细胞功能障碍、炎症和氧化应激。薯蓣皂苷元（DG）是几种植物产生的一种甾体皂苷，据报道具有抗氧化活性。本研究旨在评估薯蓣皂苷元对高脂饮食（HFD）喂养小鼠氧化应激和炎症的影响。
将30只成年雄性小鼠分为三组，包括对照组，即喂食正常饮食的小鼠；高脂饮食组，即喂食高脂饮食6周的小鼠；以及高脂饮食+薯蓣皂苷元组，即每天喂食高脂饮食和薯蓣皂苷元6周的小鼠。评估白细胞介素-6（IL-6）、肿瘤坏死因子-α（TNF-α）、丙二醛（MDA）和总抗氧化能力（TAC）的活性。研究了脂肪组织的组织病理学变化。
数据显示，薯蓣皂苷元增加了TAC活性，同时MDA水平降低。此外，DG降低了TNF和IL-6水平。补充DG后，高脂饮食引起的脂肪组织组织病理学变化得以恢复。
我们的结果表明，薯蓣皂苷元有望通过提高抗氧化剂水平、调节氧化应激和促炎细胞因子来调节肥胖，这可能预防许多疾病的发生。
