Drug Applied Research Center, Nutrition Research Center, Department of Community Nutrition, Tabriz University of Medical Sciences, Tabriz, Iran.
Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran.
Inflammation. 2017 Oct;40(5):1688-1697. doi: 10.1007/s10753-017-0610-9.
Obesity is a low-grade inflammatory disease and is associated with numerous comorbidities. The current study was aimed to evaluate the effects of vitamin D administrations on markers of inflammation and oxidative stress in adipose tissue of high-fat diet-induced obese rats. In the beginning of the study, 40 rats were divided into two groups: normal diet and high-fat diet (HFD) for 16 weeks; then, each group was subdivided into two groups including ND, ND + vitamin D, HFD, and HFD + vitamin D. Vitamin D supplementation was done for 5 weeks at 500 IU/kg dosage. Tumor necrosis factor (TNF)-α, interleukin (IL)-1β, monocyte chemoattractant protein (MCP)-1, transforming growth factor (TGF)-β and IL-6 concentrations and markers of oxidative stress including glutathione peroxidase (GPx), superoxide dismutase (SOD), malondialdehyde (MDA), and catalase (CAT) concentrations in adipose tissue of rats were determined using ELISA kits and spectrophotometry methods, respectively. Vitamin D treatment led to a significant reduction in adipose tissue TNF-α concentrations in both ND + vitamin D and HFD + vitamin D groups (P < 0.05). Adipose tissue MCP-1 concentration also reduced in HFD + vitamin D group compared with HFD group. Among markers of oxidative stress in adipose tissue, SOD and GPx concentrations significantly increased in adipose tissue of HFD + vitamin D treated group compared with other groups (P < 0.05). Reduced food intake and weight gain was also occurred after vitamin D treatment. Vitamin D improved adipose tissue oxidative stress and inflammatory parameters in obese rats. Vitamin D treatment was also associated with decreased food intake and decreased weight gain in animals under a high-fat diet. Further studies are needed to better clarify the underlying mechanisms.
肥胖是一种低度炎症性疾病，与许多合并症有关。本研究旨在评估维生素 D 给药对高脂肪饮食诱导肥胖大鼠脂肪组织炎症和氧化应激标志物的影响。在研究开始时，将 40 只大鼠分为两组：正常饮食组和高脂肪饮食组（HFD）16 周；然后，每组再分为两组，包括 ND、ND+维生素 D、HFD 和 HFD+维生素 D。以 500IU/kg 的剂量补充维生素 D 5 周。使用 ELISA 试剂盒和分光光度法分别测定大鼠脂肪组织中肿瘤坏死因子 (TNF)-α、白细胞介素 (IL)-1β、单核细胞趋化蛋白 (MCP)-1、转化生长因子 (TGF)-β 和 IL-6 浓度以及氧化应激标志物谷胱甘肽过氧化物酶 (GPx)、超氧化物歧化酶 (SOD)、丙二醛 (MDA) 和过氧化氢酶 (CAT) 的浓度。维生素 D 治疗可显著降低 ND+维生素 D 和 HFD+维生素 D 组大鼠脂肪组织中 TNF-α 的浓度（P<0.05）。与 HFD 组相比，HFD+维生素 D 组大鼠脂肪组织 MCP-1 浓度也降低。在脂肪组织氧化应激标志物中，与其他组相比，HFD+维生素 D 治疗组大鼠脂肪组织中 SOD 和 GPx 浓度显著增加（P<0.05）。维生素 D 治疗后，动物的食物摄入量和体重增加也减少。维生素 D 改善了肥胖大鼠脂肪组织的氧化应激和炎症参数。维生素 D 治疗还与高脂肪饮食动物的食物摄入量减少和体重减轻有关。需要进一步的研究来更好地阐明潜在的机制。
