The Center for Growth, Metabolism and Aging, College of Life Sciences, Sichuan University, Chengdu, China.
Department of Gastroenterology, West China Hospital, Sichuan University, Chengdu, China.
Am J Physiol Gastrointest Liver Physiol. 2022 Aug 1;323(2):G102-G113. doi: 10.1152/ajpgi.00291.2021. Epub 2022 May 31.
Inflammatory bowel diseases (IBD), including Crohn's disease (CD) and ulcerative colitis (UC), are intestinal complications characterized by chronic inflammation, autophagy abnormality, and lysosomal stress, which are derived from genetic predisposition and environmental risk factors. It is generally precepted that dietary green vegetable is beneficial for physiological homeostasis. In this study, we found that dextran sulfate sodium (DSS)-induced colitis and altered intestinal epithelia in mice were attenuated by oral administration of chlorophyllin (CHL), a water-soluble derivate of chlorophyll. In DSS-treated mice, autophagy was persistently activated in intestinal tissues and associated with bowel disorders. Conversely, supplement of CHL in diet or gavage suppressed intestinal inflammation, downregulated autophagy flux in intestinal tissue, and relieved endoplasmic reticulum stress. In vitro studies show that CHL could activate Akt and mTOR pathways, leading to downregulation of autophagic and lysosomal flux. Thus, consumption of green vegetables and chlorophyllin may be beneficial for IBD recovery in part through alleviation of inflammation and autolysosomal flux. Inflammatory bowel disease (IBD) is a chronic and recurrent gastrointestinal disease, while the etiology remains poorly understood. Dietary composition and lifestyle are crucial for pathogenesis and progression of IBD. In this study, we observed that autophagy in the intestinal tissue was persistently activated in IBD mice. Chlorophyllin (CHL), a water-soluble derivate of chlorophyll, can attenuate colitis by regulating autophagy and inflammation. Thus, consumption of green vegetables and chlorophyllin may be beneficial for IBD recovery.
炎症性肠病（IBD），包括克罗恩病（CD）和溃疡性结肠炎（UC），是肠道并发症，其特征为慢性炎症、自噬异常和溶酶体应激，这些源自遗传易感性和环境风险因素。人们普遍认为，食用绿色蔬菜对生理稳态有益。在这项研究中，我们发现，在小鼠中，葡聚糖硫酸钠（DSS）诱导的结肠炎和肠道上皮改变，可通过口服叶绿素（CHL）减轻，CHL 是叶绿素的水溶性衍生物。在 DSS 处理的小鼠中，自噬在肠道组织中持续激活，并与肠道紊乱有关。相反，饮食中或灌胃补充 CHL 可抑制肠道炎症，下调肠道组织中的自噬通量，并缓解内质网应激。体外研究表明，CHL 可以激活 Akt 和 mTOR 通路，导致自噬和溶酶体通量的下调。因此，食用绿色蔬菜和叶绿素可能有益于 IBD 的恢复，部分原因是减轻炎症和自噬溶酶体通量。炎症性肠病（IBD）是一种慢性和复发性胃肠道疾病，其病因仍不清楚。饮食成分和生活方式对 IBD 的发病机制和进展至关重要。在这项研究中，我们观察到 IBD 小鼠的肠道组织中自噬持续激活。叶绿素（CHL）是叶绿素的水溶性衍生物，可通过调节自噬和炎症来减轻结肠炎。因此，食用绿色蔬菜和叶绿素可能有益于 IBD 的恢复。
