Laboratory of Environmental Processes Engineering, University of Salah Boubnider Constantine 3, Ali Mendjli, 25000 Constantine, Algeria; Profesora Facultad de Ingeniería Agroindustrial, Universidad de Nariño (UdeNar), Pasto, Colombia.
Profesora Facultad de Ingeniería Agroindustrial, Universidad de Nariño (UdeNar), Pasto, Colombia.
J Chromatogr A. 2022 Jul 19;1675:463147. doi: 10.1016/j.chroma.2022.463147. Epub 2022 May 18.
This work reports the application of Gas Expanded Liquid (GXL) extraction to concentrate the flavonolignan fraction (silymarin) and taxifolin from Silybum marianum seeds, which have proven to be highly valuable health-promoting compounds. GXL using green solvents was used to isolate silymarin with the objective of replacing conventional methods. In one hand, the effect of different compositions of solvents, aqueous ethanol (20%, 50% or 80% (v/v)) at different CO/liquid (25, 50 and 75%) ratios, on the GXL extraction was investigated. The obtained extracts have been chemically and functionally characterized by means of UHPLC-ESI-MS/MS (triple quadrupole) and in-vitro assays such as anti-inflammatory, anti-cholinergic and antioxidant. Results revealed that the operating conditions influenced the extraction yield, the total phenolic content and the presence of the target compounds. The best obtained yield was 55.97% using a ternary mixture of solvents composed of CO:EtOH:HO (25:60:15) at 40 °C and 9 MPa in 160 min. Furthermore, the results showed that obtained extracts had significant antioxidant and anti-inflammatory activities (with best IC value of 8.80 µg/mL and 28.52 µg/mL, respectively) but a moderate anti-cholinesterase activity (with best IC value of 125.09 µg/mL). Otherwise, the concentration of silymarin compounds in extract can go up to 59.6% using the present one-step extraction method without further purification, being silybin the predominant identified compound, achieving value of 545.73 (mg silymarin/g of extract). The obtained results demonstrate the exceptional potential of GXL to extract high-added values molecules under sustainable conditions from different matrices.
本工作报道了气体膨胀液体（GXL）萃取在浓缩水飞蓟宾和圣草酚（水飞蓟素）方面的应用，水飞蓟宾和圣草酚已被证明具有高度的促进健康的价值。使用绿色溶剂的 GXL 被用于分离水飞蓟素，旨在替代传统方法。一方面，研究了不同组成的溶剂（20%、50%或 80%（v/v）乙醇水溶液）在不同 CO/液体（25、50 和 75%）比下对 GXL 萃取的影响。通过 UHPLC-ESI-MS/MS（三重四极杆）和体外抗炎、抗胆碱能和抗氧化测定等方法对获得的提取物进行了化学和功能表征。结果表明，操作条件影响萃取收率、总酚含量和目标化合物的存在。在 40°C 和 9 MPa 下，使用 CO:EtOH:HO（25:60:15）的三元混合溶剂在 160 分钟内获得了最佳的收率 55.97%。此外，结果表明，获得的提取物具有显著的抗氧化和抗炎活性（最佳 IC 值分别为 8.80 µg/mL 和 28.52 µg/mL），但抗胆碱酯酶活性中等（最佳 IC 值为 125.09 µg/mL）。此外，使用本一步提取方法，无需进一步纯化，提取物中水飞蓟素化合物的浓度可高达 59.6%，其中主要鉴定的化合物为水飞蓟宾，达到 545.73（mg 水飞蓟素/g 提取物）的值。所得结果表明，GXL 在可持续条件下从不同基质中提取高附加值分子具有特殊潜力。
