Jiang Xin-Chi, Zhang Tianyuan, Gao Jian-Qing
College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, Zhejiang 310058, PR China; Dr. Li Dak Sum & Yip Yio Chin Center for Stem Cell and Regenerative Medicine, Zhejiang University, Hangzhou, Zhejiang 310058, PR China.
College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, Zhejiang 310058, PR China; Dr. Li Dak Sum & Yip Yio Chin Center for Stem Cell and Regenerative Medicine, Zhejiang University, Hangzhou, Zhejiang 310058, PR China; Hangzhou Institute of Innovative Medicine, Zhejiang University, Hangzhou, Zhejiang 310058, PR China; Department of Pharmacy, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310009, PR China.
Adv Drug Deliv Rev. 2022 Aug;187:114324. doi: 10.1016/j.addr.2022.114324. Epub 2022 May 29.
Exosomes and biomimetic vesicles are widely used for gene delivery because of their excellent gene loading capacity and stability and their natural targeting delivery potential. These vesicles take advantages of both cell-based bioactive delivery system and synthetical lipid-derived nanovectors to form crossover characteristics. To further optimize the specific targeting properties of crossover vesicles, studies of their in vivo fate and various engineering approaches including nanobiotechnology are required. This review describes the preparation process of exosomes and biomimetic vesicles, and summarizes the mechanism of loading and delivery of nucleic acids or gene editing systems. We provide a comprehensive overview of the techniques employed for preparing the targeting crossover vesicles based on their cellular uptake and targeting mechanism. To delineate the future prospects of crossover vesicle gene delivery systems, various challenges and clinical applications of vesicles have also been discussed.
外泌体和仿生囊泡因其出色的基因装载能力、稳定性以及天然的靶向递送潜力而被广泛用于基因递送。这些囊泡兼具基于细胞的生物活性递送系统和合成脂质衍生纳米载体的优势，形成了交叉特性。为进一步优化交叉囊泡的特异性靶向特性，需要研究其体内命运以及包括纳米生物技术在内的各种工程方法。本综述描述了外泌体和仿生囊泡的制备过程，并总结了核酸或基因编辑系统的装载和递送机制。我们基于其细胞摄取和靶向机制，全面概述了用于制备靶向交叉囊泡的技术。为了描绘交叉囊泡基因递送系统的未来前景，还讨论了囊泡的各种挑战和临床应用。
