Department of Electrical and Computer Engineering, University of Maryland, College Park, MD, 20742, USA; Institute for Research in Electronics and Applied Physics, University of Maryland, College Park, MD, 20742, USA.
Vaccine and Infectious Diseases Division, Fred Hutchinson Cancer Research Center, Seattle, WA, 98109, USA.
Biosens Bioelectron. 2022 Feb 1;197:113803. doi: 10.1016/j.bios.2021.113803. Epub 2021 Nov 17.
We report the rapid detection of SARS-CoV-2 in infected patients (mid-turbinate swabs and exhaled breath aerosol samples) in concentrations as low as 60 copies/mL of the virus in seconds by electrical transduction of the SARS-CoV-2 S1 spike protein antigen via SARS-CoV-2 S1 spike protein antibodies immobilized on bilayer quasi-freestanding epitaxial graphene without gate or signal amplification. The sensor demonstrates the spike protein antigen detection in a concentration as low as 1 ag/mL. The heterostructure of the SARS-CoV-2 antibody/graphene-based sensor is developed through a simple and low-cost fabrication technique. Furthermore, sensors integrated into a portable testing unit distinguished B.1.1.7 variant positive samples from infected patients (mid-turbinate swabs and saliva samples, 4000-8000 copies/mL) with a response time of as fast as 0.6 s. The sensor is reusable, allowing for reimmobilization of the crosslinker and antibodies on the biosensor after desorption of biomarkers by NaCl solution or heat treatment above 40 °C.
我们报告了一种快速检测 SARS-CoV-2 的方法，通过将 SARS-CoV-2 S1 刺突蛋白抗原固定在双层准自由站立外延石墨烯上的 SARS-CoV-2 S1 刺突蛋白抗体的电转导，可在 60 拷贝/毫升的低病毒浓度下，在数秒内检测到受感染患者（中鼻甲拭子和呼出的气溶胶样本）中的 SARS-CoV-2。该传感器无需门控或信号放大，即可在低至 1 ag/mL 的浓度下检测到刺突蛋白抗原。该 SARS-CoV-2 抗体/基于石墨烯的传感器的异质结构是通过一种简单且低成本的制造技术开发的。此外，集成到便携式测试单元中的传感器能够以最快 0.6 s 的响应时间区分 B.1.1.7 变体阳性样本和感染患者（中鼻甲拭子和唾液样本，4000-8000 拷贝/毫升）。该传感器是可重复使用的，允许在通过 NaCl 溶液或高于 40°C 的热处理解吸生物标志物后，重新固定交联剂和抗体在生物传感器上。
