Guangdong Ocean University, Fishery College, 524088 Zhanjiang, China; Pearl Breeding and Processing Engineering Technology Research Centre of Guangdong Province, Zhanjiang 524088, China; Guangdong Science and Innovation Center for Pearl Culture, Zhanjiang 524088, China; Guangdong Provincial Engineering Laboratory for Mariculture Organism Breeding, Zhanjiang 524088, China; Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, Zhanjiang, China.
Guangdong Ocean University, Fishery College, 524088 Zhanjiang, China.
Comp Biochem Physiol Part D Genomics Proteomics. 2022 Sep;43:101004. doi: 10.1016/j.cbd.2022.101004. Epub 2022 May 18.
MicroRNAs (miRNAs) can efficiently regulate gene expression at intracellular and extracellular levels. Plant-derived miRNAs are highly enriched in animal haemolymph and regulate mammalian gene expression. However, evidence for food-derived miRNAs in Mollusca species is lacking. In this study, we fed the microalga Nannochloropsis oculata to the pearl oyster Pinctada fucata martensii and detected dietary miRNAs in exosomes isolated from the haemolymph by RNA-seq. In total, 273 endogenous miRNAs were identified in all biological replicates. We identified 23 microalgae-derived miRNAs in the exosomes of pearl oyster haemolymph. Most microalgae-derived miRNAs showed high expression levels in both exosomes and microalgae and exhibited apparent variation among individuals. These food-derived miRNAs were predicted to participate in endocytosis, apoptosis, signal transduction, energy metabolism, and biomineralization by targeting multiple genes. These findings demonstrated the cross-kingdom transport of miRNAs from microalgae to bivalves and provide insights into novel nutrient transmission through the food chain.
微小 RNA（miRNAs）可以在细胞内和细胞外水平高效调节基因表达。植物来源的 miRNAs 在动物血淋巴中高度富集，并调节哺乳动物基因表达。然而，软体动物物种中食物来源的 miRNAs 的证据尚缺乏。在这项研究中，我们用微藻眼点拟微绿球藻喂食珍珠贝马氏珍珠贝，并通过 RNA-seq 从血淋巴中分离的外泌体中检测膳食 miRNA。在所有生物学重复中，共鉴定出 273 个内源性 miRNAs。我们在珍珠贝血淋巴外泌体中鉴定出 23 种微藻衍生的 miRNAs。大多数微藻衍生的 miRNAs 在外泌体和微藻中均表现出高表达水平，并在个体间表现出明显的差异。这些食物来源的 miRNAs 被预测通过靶向多个基因参与胞吞作用、细胞凋亡、信号转导、能量代谢和生物矿化。这些发现表明 miRNA 从微藻到双壳类动物的跨物种运输，并为通过食物链进行新的营养物质传递提供了新的视角。
