Department of Conservative Dentistry and Endodontics, Saveetha Dental College & Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, Tamil Nadu, India.
Department of Anatomy, Saveetha Dental College & Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, Tamil Nadu, India.
Indian J Dent Res. 2021 Oct-Dec;32(4):423-431. doi: 10.4103/ijdr.IJDR_436_19.
An ideal irrigant should exhibit good antimicrobial activity along with smear removal. Various plant extracts have been used for disinfection of the root canal system. The present study aims in evaluating the tissue response and systemic toxicity of ethanolic extract of Morinda citrifolia versus Morinda citrifolia fresh fruit juice in an animal model.
A total 48 female adult wistar albino rats were randomly allocated into 4 groups as Group I- 3% sodium hypochlorite (n = 12), Group II- Saline (Control) (n = 12), Group III- ethanolic extract of Morinda citrifolia (n = 12), Group IV- Morinda citrifolia Fresh Fruit Juice (n = 12). A trough was made in the periapical bone, and the test sample was placed in the respective groups. The animals were euthanized for 7 day, 14 day and 30 day and the tissue specimen was prepared for histopathological evaluation, and serum analysis was done for oxidative stress markers, liver and renal function tests.
Serum analysis exhibited significant elevation of Alkaline Phosphatase (ALP), Glutathione Peroxidase (GPX) and Glutathione Reductase (GR) and histopathology showed a higher inflammatory response at 7 and 14 days in group II and group III (p < 0.05), as compared to the other groups. At the 30 day time interval, there was no statistically significant difference in serum analysis and inflammatory response, in all the four groups analysed (p > 0.05).
Ethanolic extract of Morinda citrifolia was not biocompatible and more cytotoxic than a Morinda citrifolia fresh fruit juice. So, fresh fruit juice of Morinda citrifolia can be a preferred choice of natural root canal irrigant, with least possible cytotoxic effects as compared to the ethanolic extract.
理想的冲洗液应具有良好的抗菌活性和清除玷污层的能力。各种植物提取物已被用于根管系统的消毒。本研究旨在评估印度醋栗乙醇提取物与新鲜印度醋栗果汁对动物模型的组织反应和全身毒性。
将 48 只成年雌性 Wistar 白化大鼠随机分为 4 组：I 组-3%次氯酸钠（n=12）、II 组-生理盐水（对照组）（n=12）、III 组-印度醋栗乙醇提取物（n=12）、IV 组-印度醋栗新鲜果汁（n=12）。在根尖骨上制作一个槽，将测试样品放置在相应的组中。动物在第 7 天、第 14 天和第 30 天被安乐死，然后制备组织标本进行组织病理学评估，并进行血清分析以检测氧化应激标志物、肝功能和肾功能测试。
血清分析显示碱性磷酸酶（ALP）、谷胱甘肽过氧化物酶（GPX）和谷胱甘肽还原酶（GR）显著升高，组织病理学显示第 7 天和第 14 天 II 组和 III 组的炎症反应更高（p<0.05），与其他组相比。在 30 天的时间间隔内，四组的血清分析和炎症反应均无统计学差异（p>0.05）。
印度醋栗乙醇提取物不具有生物相容性，比印度醋栗新鲜果汁的细胞毒性更强。因此，与乙醇提取物相比，新鲜的印度醋栗果汁可以作为天然根管冲洗剂的首选，其细胞毒性最小。
