Department of Microbiology, Tehran University of Medical Sciences, Tehran, Iran.
Dental Research Center, Dentistry Research Institute, Tehran University of Medical Sciences, Tehran, Iran.
ScientificWorldJournal. 2022 May 19;2022:3613345. doi: 10.1155/2022/3613345. eCollection 2022.
This study aimed to evaluate the antimicrobial effect of curcumin doped poly-lactic-co-glycolic acid nanoparticles (Cur-PLGA-Nps)-mediated antimicrobial photodynamic therapy (aPDT), as well as the probiotics on in rats with fixed orthodontic appliances. Orthodontic appliances were ligated to the right maxillary of the rats. After the oral colonization of , the rats were then treated in four groups including Cur-PLGA-Nps, light-emitting diode, Cur-PLGA-Nps-mediated aPDT, and probiotic (). After that, the counts and the gene expression of were determined on days 4, 7, 15, and 30. Probiotic and Cur-PLGA-Nps-mediated aPDT groups significantly reduced the count of in a time-dependent manner ( < 0.05). So, probiotics and Cur-PLGA-Nps-mediated aPDT were able to reduce more than other groups on the 30 day. Also, there was no considerable difference between Cur-PLGA-Nps-mediated aPDT and probiotic groups in bacterial growth inhibition. The expression level of gene was significantly downregulated at all-time intervals after exposure to Cur-PLGA-Nps-mediated aPDT compared with untreated bacteria ( < 0.05). According to the results, simultaneous use of Cur-PLGA-Nps-mediated aPDT and probiotic therapeutic approaches can be suggested to increase effectiveness.
本研究旨在评估姜黄素掺杂聚乳酸-共-羟基乙酸纳米粒子（Cur-PLGA-Nps）介导的光动力抗菌疗法（aPDT）以及益生菌对固定正畸大鼠的抗菌作用。将正畸矫治器结扎到大鼠的右上颌。在 口腔定植后，将大鼠分为四组进行治疗，包括 Cur-PLGA-Nps、发光二极管、Cur-PLGA-Nps 介导的 aPDT 和益生菌（）。然后，在第 4、7、15 和 30 天测定 计数和 基因的表达。益生菌和 Cur-PLGA-Nps 介导的 aPDT 组可随时间依赖性地降低 的计数（<0.05）。因此，益生菌和 Cur-PLGA-Nps 介导的 aPDT 组在第 30 天时比其他组更能减少 的生长。此外，在细菌生长抑制方面，Cur-PLGA-Nps 介导的 aPDT 组和益生菌组之间没有明显差异。与未经处理的细菌相比，Cur-PLGA-Nps 介导的 aPDT 处理后所有时间间隔的 基因表达水平均显著下调（<0.05）。根据结果，建议同时使用 Cur-PLGA-Nps 介导的 aPDT 和益生菌治疗方法以提高疗效。
