Lin Zhizhong, Chen Lin, Wu Tingting, Zhang Yiping, Huang Xinyi, Chen Yuanmei, Chen Junqiang, Xu Yuanji
Department of Radiation Oncology, Fujian Medical University Cancer Hospital, Fujian Cancer Hospital, Fuzhou, China.
The School of Nusing, Fujian Medical University, Fuzhou, China.
Front Genet. 2022 May 11;13:872026. doi: 10.3389/fgene.2022.872026. eCollection 2022.
In the study, we aimed to explore and analyze the potential function of SPOC Domain Containing 1 (SPOCD1) in esophageal squamous cell carcinoma (ESCC). We performed a comprehensive analysis of gene expression of SPOCD1 and its corresponding clinicopathological features in ESCC. In particular, the correlation between SPOCD1 and ESCC was evaluated using a wide range of analysis tools and databases, including TCGA, GTEx, GenePattern, CellMiner, GDSC, and STRING datasets. Different bioinformatics analyses, including differential expression analysis, mutation analysis, drug sensitivity analysis, function analysis, pathway analysis, co-expression network analysis, immune cell infiltration analysis, and survival analysis, were carried out to comprehensively explore the potential molecular mechanisms and functional effects of SPOCD1 on the initiation and progression of ESCC. The expression of SPOCD1 was upregulated in ESCC tissues compared to those in normal tissues. In the high SPOCD1 expression group, we found apparent mutations in TP53, TTN, and MUC16 genes, which were 92, 36, and 18%, respectively. GO and KEGG enrichment analysis of SPOCD1 and its co-expressed genes demonstrated that it may serve as an ESCC oncogene by regulating the genes expression in the essential functions and pathways of tumorigenesis, such as glycosaminoglycan binding, Cytokine-cytokine receptor interaction, and Ras signaling pathway. Besides, the immune cell infiltration results revealed that SPOCD1 expression was positively correlated with Macrophages M0 and Mast cells activated cells, and negatively correlated with plasma cells and T cells follicular helper cell infiltration. Finally, ESCC patients with high expression of SPOCD1 indicated poor overall survival. qRT-PCR demonstrated that the SPOCD1 expression in ESCC tissues was significantly higher than adjacent tissues ( < 0.001). Our study indicated that SPOCD1 was increased in ESCC tissues. The current data support the oncogenic role of SPOCD1 in the occurrence and development of ESCC. Most importantly, SPOCD1 might be an independent prognostic factor for ESCC patients.
在本研究中，我们旨在探索和分析含SPOC结构域蛋白1（SPOCD1）在食管鳞状细胞癌（ESCC）中的潜在功能。我们对ESCC中SPOCD1的基因表达及其相应的临床病理特征进行了全面分析。特别是，使用了多种分析工具和数据库，包括TCGA、GTEx、GenePattern、CellMiner、GDSC和STRING数据集，来评估SPOCD1与ESCC之间的相关性。进行了不同的生物信息学分析，包括差异表达分析、突变分析、药物敏感性分析、功能分析、通路分析、共表达网络分析、免疫细胞浸润分析和生存分析，以全面探索SPOCD1对ESCC发生和进展的潜在分子机制和功能影响。与正常组织相比，ESCC组织中SPOCD1的表达上调。在SPOCD1高表达组中，我们发现TP53、TTN和MUC16基因存在明显突变，分别为92%、36%和18%。对SPOCD1及其共表达基因的GO和KEGG富集分析表明，它可能通过调节肿瘤发生的基本功能和通路中的基因表达，如糖胺聚糖结合、细胞因子-细胞因子受体相互作用和Ras信号通路，而作为ESCC癌基因。此外，免疫细胞浸润结果显示，SPOCD1表达与M0巨噬细胞和活化肥大细胞呈正相关，与浆细胞和滤泡辅助性T细胞浸润呈负相关。最后，SPOCD1高表达的ESCC患者总体生存率较差。qRT-PCR表明，ESCC组织中SPOCD1的表达显著高于相邻组织（<0.001）。我们的研究表明，ESCC组织中SPOCD1增加。目前的数据支持SPOCD1在ESCC发生发展中的致癌作用。最重要的是，SPOCD1可能是ESCC患者的一个独立预后因素。
