Antiviral Effect of Polyphenolic Substances in against HSV-2 Infection Using and Approaches.

BACKGROUND

type 2 (HSV-2) infestation was the most widespread STD (sexually transmitted diseases) among humans and was the leading cause of infectious recurrent genital herpes. Existing therapies against HSV-2 did incompletely restrain the comeback of activated HSV-2 infestation. had long been used as traditional Chinese medicine for treating the diseases owing to its anti-inflammatory and antiviral effects. Herein, the study was designed to investigate the antiviral activity of and its potential effect in regulating the host's immune response.

METHODS

To identify the stage of infection at which the compounds inhibited HSV-2, we performed virucidal, therapeutic, and prophylactic assays. The antiviral efficacy was evaluated by the analysis of viral components HSV-2 and . The antiviral activities of these compounds were also evaluated by phenotypic analysis, such as cell proliferation and apoptosis. Molecular docking studies on candidate compounds were done to indicate binding interactions between the compounds and adopted compound targets.

RESULTS

Quercetin, corilagin, and geraniin inhibited the replication of HSV-2, with geraniin showing greater TI. The obtained IC value of quercetin was 204.7 M and TI (IC/EC) was 5.1, whereas the obtained IC value of corilagin was 118.0 g/ml and TI was 4.05. Geraniin exhibited prominent antiviral activity with an IC of 212.4 M and an EC of 18.37 M, resulting in a therapeutic index (TI) of 11.56. Geraniin showed important virucidal activity through blocking viral attachment. Compared with the virus group, the apoptosis rates in quercetin-, corilagin-, and geraniin-treated groups were significantly decreased ( < 0.001).The expressions at the transcription genes of virus own replication key factors (including HSV-2 and ) and cytokines (including ) of infected cells treated with quercetin, corilagin, and geraniin were inhibited. The approaches demonstrated a high number of potential strong intermolecular interactions as hydrogen bonds between geraniin, corilagin, and the activity site of HSV-2 . Molecular docking studies demonstrated the effects of corilagin by targeting .

CONCLUSIONS

Together, these results highlighted the importance of treatment in HSV-2 infection and underscored its therapeutic potential. However, additional and research was required to validate our findings.