Sathyanarayanan Saikumar, Sreeja Puthanpura Sasidharan, Arunachalam Karuppusamy, Parimelazhagan Thangaraj
Department of Botany, PSG College of Arts and Science, Coimbatore 641014, Tamil Nadu, India.
Bioprospecting Laboratory, Department of Botany, Bharathiar University, Coimbatore 641046, Tamil Nadu, India.
Evid Based Complement Alternat Med. 2022 May 18;2022:4328571. doi: 10.1155/2022/4328571. eCollection 2022.
Humans have been using herbs to prevent and cure various ailments since antiquity, and is a significant medicinal plant known for its wide ethnobotanical uses. Although the plant is known to treat ulcers, there is no significant scientific validation. The present study aimed to assess the acute toxicity, subacute toxicity, and antiulcer properties of the leaf methanol extract of (IWL). In the subacute study, the extracts were given orally at 100, 200, and 400 mg/kg doses for 28 days, and we analyzed the biochemical and histological parameters to evaluate the toxicity of IWL. Two different models were assessed to explore antiulcer properties, such as indomethacin- and ethanol-induced ulcer model. Ulcer areas and ulceration percentage histopathology of the stomach were used to study the efficacy of extracts. The acute toxicity study showed that IWL was safe to the maximum dose of 2000 mg/kg body weight. In a subacute toxicity study, the oral administration of IWL did not produce any mortality in the tested animals. The analysis of haematological, liver biochemical, kidney profile, lipid profile, and antioxidant parameters depicted that all the values were within the control limits after the experimental period and were considered nontoxic to animals. Additionally, the antiulcer study demonstrated a positive response of IWL in a dose-related manner (indomethacin- and ethanol-induced models). Macroscopic analysis showed that pretreatment with leaf methanol extract significantly reduced the gastric lesion and decreased the ulceration area (14.52 mm), demonstrating superior results to the positive control group (27.71 mm). The histopathological analysis revealed that pretreatment with a high dose of 400 mg/kg of leaf methanol extract and positive control group (omeprazole) markedly protected pathological effects, and the gastric mucosa appeared normal. In conclusion, has solid nontoxic potential as a promising native herb for an integral therapy for the treatment of ulcers.
自古以来，人类就一直使用草药来预防和治疗各种疾病，[植物名称]是一种重要的药用植物，以其广泛的民族植物学用途而闻名。尽管已知这种植物可治疗溃疡，但尚无显著的科学验证。本研究旨在评估[植物名称]叶甲醇提取物（IWL）的急性毒性、亚急性毒性和抗溃疡特性。在亚急性研究中，以100、200和400mg/kg的剂量口服提取物，持续28天，我们分析了生化和组织学参数以评估IWL的毒性。评估了两种不同的模型以探究抗溃疡特性，如吲哚美辛和乙醇诱导的溃疡模型。胃的溃疡面积和溃疡百分比组织病理学用于研究提取物的疗效。急性毒性研究表明，IWL在最大剂量为2000mg/kg体重时是安全的。在亚急性毒性研究中，口服IWL在受试动物中未产生任何死亡。血液学、肝脏生化、肾脏指标、血脂指标和抗氧化参数分析表明，实验期后所有值均在控制范围内，被认为对动物无毒。此外，抗溃疡研究表明IWL以剂量相关的方式呈现阳性反应（吲哚美辛和乙醇诱导的模型）。宏观分析表明，用[植物名称]叶甲醇提取物预处理可显著减少胃部病变并减小溃疡面积（14.52mm），显示出优于阳性对照组（27.71mm）的结果。组织病理学分析显示，用400mg/kg的高剂量[植物名称]叶甲醇提取物预处理和阳性对照组（奥美拉唑）可显著保护病理效应，胃黏膜外观正常。总之，[植物名称]作为一种有前景的本土草药，在溃疡综合治疗中具有可靠的无毒潜力。
